Use of Capillary Electrophoresis for Detection of Hemoglobinopathies in Individuals Referred to Health Centers in Masjed-Soleiman
Journal Title: Iranian Journal of Blood and Cancer - Year 2017, Vol 9, Issue 3
Abstract
Background: Hemoglobinopathies are the commonest single gene disorder in human that affect hemoglobin production and function that occur when mutations alter the amino acid sequence of globin chains. The purpose of the present study was to evaluate the prevalence of hemoglobninopathies detected by capillary electrophoresis method in individuals referred to Masjed-Soleiman health centers by capillary electrophoresis method. Methods: This study was carried out on 394 individuals referred to Masjed-Soleiman health centers during 2015-2016. Blood samples were collected in EDTA vacutainer tubes, then CBC including blood indexes (MCV, MCH), level of Hemoglobin A, Hb F, Hb A2 and other hemoglobins were evaluated by Sebia minicap (France) and also genetic tests applied for them to confirm results that were aqcuired by capillary electrophoresis method. Results: 77 (19.5%) subjects had HbA2 ≥3.5%, thus were classified as beta thalassemia carrier and 3.3%, 2.5%, 1.5% and 0.5% of the individuals were heterozygote for Hb S, Hb D, Hb C and Hb Bart, respectively. Results of the genetic analysis showed the mutations in these subjects; cd36-37(-T) was the most frequent mutation in beta thalassemia carriers in this geographic region. Conclusion: This study showed high frequency of beta thalassemia mutations in the geographic region of Masjed-Soleiman (19.5), and 7.85% of the individuals had hemoglobin variants including Hb S, Hb D and Hb C detected by capillary electrophoresis. Capillary electrophoresis could be a considerable method for detection of hemoglobinopathies.
Authors and Affiliations
Seyedeh Moloud Rasouli Ghahfarokhi, Fatemeh Asadi, Narges Obeidi
Keywords
Related Articles
- EP ID EP245882
- DOI -
- Views 98
- Downloads 0
Askin Tumor Presenting as Superior Vena Cava Syndrome
This article has no abstract.
Pulmonary Capillary Leak Syndrome
This article has no abstract.
Conversion from Platelet-Rich Plasma Platelet Production to Buffy Coat Platelet Component Production: Benefits and Limitations
Blood transfusion centers are under considerable pressure to produce platelet concentrates with high quality and safety due to the short shelf life of 3-5 days as well as possible bacterial and viral contaminations. For...
Effect of Pamidronate on Osteoporosis in Patients with β-Thalassemia Major
Background: β-thalassemia major is a hereditary life threatening anemia which requires regular blood transfusion. Clinical symptoms of the disease are growth retardation, pallor, jaundice and skeletal alternations. The v...
Microparticle Formation and Platelet Shrinkage in Type-I Glanzmman Thrombasthenia Platelets
Background: Activated normal platelets undergo many biochemical and morphological changes, some of which are apoptotic. Platelet derived microparticles and shrinked platelets as hallmark of platelet activation and apopto...