Use of chicken (Gallus gallus) serum as a costly replacement for the fetal calf serum in cultivation of promastigotes ofLeishmania infantum
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2013, Vol 3, Issue 3
Abstract
Objective: To evaluate the effectiveness of chicken serum as an alternative to fetal calf serum (FCS) in the culture of Leishmania. Methods: In the present work, the efficacy of chicken serum was evaluated in the cultivation of promastigotes of Leishmania infantum (L. infantum), by assassing the parasite growth and replication pattern after adding 5%-10% chicken serum to the RPMI-1640 medium. Results: The 10% chicken serum enriched culture medium, supported the growth of L. infantum. Conclusions: According to our finding, the chicken serum can be used for primary and mass cultivation of L. infantum is an effective, easy available and low-cost replacement for FCS.
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- DOI 10.1016/S2222-1808(13)60034-1
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