Use of chicken (Gallus gallus) serum as a costly replacement for the fetal calf serum in cultivation of promastigotes ofLeishmania infantum
Journal Title: Asian Pacific Journal of Tropical Disease - Year 2013, Vol 3, Issue 3
Abstract
Objective: To evaluate the effectiveness of chicken serum as an alternative to fetal calf serum (FCS) in the culture of Leishmania. Methods: In the present work, the efficacy of chicken serum was evaluated in the cultivation of promastigotes of Leishmania infantum (L. infantum), by assassing the parasite growth and replication pattern after adding 5%-10% chicken serum to the RPMI-1640 medium. Results: The 10% chicken serum enriched culture medium, supported the growth of L. infantum. Conclusions: According to our finding, the chicken serum can be used for primary and mass cultivation of L. infantum is an effective, easy available and low-cost replacement for FCS.
Detection of Mycobacterium marinum in clinically asymptomatic Siamese fighting fish (Betta splendens) from ornamental fih shops in Chiang Mai Province, Thailand
Objective: To detect Mycobacterium marinum (M. marinum) infections in healthy Siamese fighting fish from ornamental fish shops in Chiang Mai, Thailand. Methods: Mycobacterium spp. were isolated from 380 internal organs o...
Evaluation of CA-125 and other biochemical parameters in premenopausal and postmenopausal women with ovarian cancer: a hospital based study from Western Nepal
Objective: To assess the status of carbohydrate antigen 125 (CA-125) and other biochemical parameters in premenopausal and postmenopausal ovarian cancer patients. Methods: Total 64 cases, out of which 43 premenopausal an...
Tuberculous brain abscess-Case report
In spite of recent advances in understanding of disease, tuberculosis still remains a major health problem, particularly in developing countries. Central nervous system tuberculosis may present as commonly encountered tu...
A review of the health effects and uses of drugs of plant licorice (Glycyrrhiza glabra L.) in Iran
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Phenolics composition and antidiabetic property of Brachystegia eurycoma seed flur in high-fat diet, low-dose streptozotocin-induced type 2 diabetes in rats
Objective: To quantify some major pharmacologically important flavonoids and phenolic acids in Brachystegia eurycoma seed flour (BESF) and evaluate its antidiabetic activity in type 2 diabetic rats. Method: Flavonoids an...