Use of cytochrome oxidase 1 gene region: a molecular tool for the domestic and wildlife industry in Kenya
Journal Title: Scientific Journal of Animal Science - Year 2014, Vol 3, Issue 3
Abstract
Illegal substitution of meat products by traders either as closely related domestic species or as bush meat is a common occurrence in various parts of Kenya. This has implications on biosafety, food safety and consumer confidence and subsequently the meat and meat products industry both locally and export. In recent times, use of molecular techniques have seen increased application in wildlife conservation through conservation genetics in areas such as population genetics, evolutionary genetics, molecular ecology and wildlife forensics. We used DNA of the Cytochrome C Oxidase 1 gene region as a bar-coding technique for species identification. The accuracy of CO1 as a marker was tested using five known samples of wildlife species.Retail meat product substitution and bushmeat prevalence was estimated from 99 unknown meat samples that were randomly collected from meat traders in Nakuru County. The study validated the use of CO1 marker for species identification and illustrated use of the marker in identification of unknown tissue samples collected from the market survey.
Authors and Affiliations
D. Mbugua*, C. N. Kimwele, J. O. Jung’a, E. Gatebe, F. Gakuya, M. J. Kinuthia, M. M. Mugambi, E. S. Kwamboka
Keywords
Related Articles
- EP ID EP683
- DOI 10.14196/sjas.v3i3.1137
- Views 465
- Downloads 23
Opportunities and needs in development of smallholder goat production systems in Africa
A detailed diagnosis of the dynamics of smallholder goat farming systems will guide in any of livelihood intervention programs which takes into account the important socioeconomic issues towards improving the standard...
Polymerase chain reaction detection of candidatus liberibacter asiatic associated with citrus huanglonbing
Polymerase chain reaction diagnosis of Candidatus liberibacter asiatic associated with citrus Huanglonbing disease is molecular technique which is used for detection of disease when pathogen present is very low concent...
Serum and urine analyte comparison between llamas and alpacas fed three forages
Serum and urine analytes were measured in 4 healthy adult llama and alpaca geldings housed in metabolism crates and fed three diets consisting of alfalfa (AH), barley (BH) or grass (GH) hay and water ad libitum. This com...
Prospects for utilization of the relationship between zoometrical measurements and performance traits for poultry and livestock genetic improvement in developing countries
The preceding review looks at the prospects for utilization of the relationship between zoometrical measurements and performance traits for poultry and livestock genetic improvement in developing countries. Improvement...
Influence of baobab fruit in the diet on intake, milk yield and milk composition in Red Sokoto goats
Baobab fruit (Adansonia digitata) is a nutritious but grossly underutilized food resource with great potentials for improving the productivity of milking goats in Nigeria. Twenty four Red Sokoto goats fed diets containin...