Use of dried blood spot as an alternative method to estimate serum triglyceride in field studies
Journal Title: International Journal of Clinical Biochemistry and Research - Year 2016, Vol 3, Issue 1
Abstract
Introduction: Cardiovascular diseases are showing an increasing trend in both developing as well as developed countries. Raised triglycerides is considered as an important risk factor of cardio vascular diseases. Collection, storage and transportation of the blood samples from the field becomes a major limiting factor in areas lacking modern laboratory facilities. In such situations, dry blood or serum spots can become an alternative for the estimation of triglyceride. The objectives of our study was to evaluate the use of dry blood or dry serum spot to estimate triglyceride levels as an alternative method to estimate serum triglyceride and also to study the effect of storage time and storage temperature on the triglyceride content in the dry blood and serum spots. Methodology: Blood samples were collected from 100 study participants. Seven spots each of blood and serum from these samples were made on Whatman paper 3. After drying, one dried spot each from blood and serum was analysed on same day. Three of blood and three of serum dried spots were kept at room temperature and remaining at a temperature of 2-8° in separate resealable bags which were analysed on days 7, 15 and 30. Triglyceride was measured by Glycerol-3-phosphate oxidase-Peroxidase method. For estimating triglycerides in the eluate, 100 microlitre of the extract from the test-tube was added to 1 ml of the commercially available enzymatic reagent kit and incubated at 37°C for 15 min, and the absorbance was measured at 500 nm using a spectrophotometer. The data collected was analysed by Pearson’s correlation using SPSSv15. Results: Study showed a good correlation between the serum triglyceride values and the dry serum or blood spot triglyceride values. Dried serum spot triglyceride was found to be stable for about 15 days at room temperature and 7 days at 2-8°C with good correlation with serum value. The triglyceride concentration in the dry blood spots remained stable for 7 days at both room temperature and at 2-8°C. Conclusion: Dry blood/serum spots can be used as an alternative to serum for the estimation of triglyceride level which can be used to estimate various biochemical parameters at community level in remote areas which lack modern laboratory facilities.
Authors and Affiliations
Hamsa M, Raghunath H, Kruthi BN, T Sibi Mandela
Keywords
Related Articles
- EP ID EP242121
- DOI -
- Views 115
- Downloads 0
The synergistic role of CKMB mass and high sensitivity Troponin T in the diagnosis of Acute Coronary Syndrome
Aim: To determine the synergistic role of CKMB mass (CKMBM) and high sensitivity Troponin T (hs-cTnT) in the diagnosis of Acute Coronary Syndrome (ACS). Study Design: This was a cross-sectional study comprising of 140 in...
Altered erythrocyte surface electric charge in β- thalassemia: By Alcian Blue Dye binding
Aim: To evaluate erythrocyte surface electric charge in β- thalassemia by using Alcian Blue dye binding. Objective: Estimate the binding of AB dye to Sialic acid on the surface of thalassemia and normal, healthy erythroc...
Association of serum MDA with Magnesium, Calcium and Zinc levels in thalassemic and healthy children
Introduction: The thalassemias are group of haemoglobin disorders, initially found in 1925 in United States and Italy. It is the genetic disorder which passed down through families. In this disease deficient haemoglobin...
Correlation of anthropometric parameters with lipid profile in first year medical students
Introduction: Lifestyle related risk factors are prone to increase the burden of cardiovascular diseases. Thus, the study was carried out in the medical students who are more prone to develop lifestyle diseases because o...
Comparison of electrolyte levels in serum and plasma
Introduction: Measurement of electrolytes plays a significant role in the management of critically ill patients. The accuracy of electrolyte measurement depends on a number of pre-analytical variables. Electrolytes are t...