Use of Low Molecular Weight Antigens of Mycobacterium tuberculosis as a New Skin Test for Specific Diagnosis of Tuberculosis
Journal Title: مجله علمی- پژوهشی دانشگاه علوم پزشکی قم - Year 2018, Vol 12, Issue 1
Abstract
Background and Objectives: Tuberculosis (TB) is an infectious disease and about one-third of the world population are infected with latent TB infection. Active TB infection may develop in 5 to10 percent of individuals with latent infection; therefore, detection of latent infection is of great importance in the control of the disease. The aim of this study was to develop a specific skin test based on low molecular weight proteins secreted by M. tuberculosis for detection of M. tuberculosis infection. Methods: At this study, after extraction of secreted proteins from short-term culture filtrate of M. tuberculosis, low molecular weight proteins, were purified by Sephadex-G75 gel chromatography. For skin test, several groups of guinea pigs were sensitized with M. tuberculosis, M. bovis BCG, and M. avium strains, and then skin test were performed using purified proteins and PPD. Results: According to skin test data, unlike PPD, purified proteins showed a positive response in animals exposed to M. tuberculosis, but no significant skin responses was observed in the guinea pigs sensitized with M. bovis BCG and M. avium strains. Conclusion: The results of this study demonstrated that new skin test is specific compared to PPD in the detection of M. tuberculosis infection, and could be replaced as a more specific skin test for detection of TB in large animals or human.
Authors and Affiliations
Davoud Davoud, Nader Nader
Keywords
Related Articles
- EP ID EP467979
- DOI 10.29252/qums.12.1.63
- Views 56
- Downloads 0
The Effect of Decreased Activity in the Form of Neuropathic Pain on GSK-3β Gene Expression in Sciatic Nerve Fiber of Male Wistar Rats
Background and Objectives: Studies have shown that impairment in kinases in the nervous system is related to many neurodegenerative disease. Glycogen synthase kinase-3β (GSK-3β) is a serine/threonine kinase that particip...
Antibacterial Activity of Rumex cyprius Seeds Against Escherichia coli and Staphylococcus aureus
Background and Objectives: The infections caused by Escherichia coli and Staphylococcus aureus resistant strains often originate from hospitals, and their prevalence is increasing worldwide. Therefore, many efforts are b...
تأثیر 10 هفته تمرین مقاومتی بر سطح سرمی میوستاتین و ترکیب بدنی در نوجوانان چاق
زمینه و هدف: مطالعات، نشان‌دهنده نقش تنظیمی منفی میوستاتین در رشد عضله اسکلتی است. در مطالعه حاضر تأثیر 10 هفته تمرین مقاومتی بر تغییرات سطح سرمی میوستاتین و ترکیب بدنی در نوجوانان چاق بررسی گردید. روش بررسی: در این مطا...
Comparison of the Viewpoints of Midwives Working in Teaching Hospitals and Health Centers in Tehran City about Management Factors Associated with Midwifery Errors (Iran)
Background and Objectives: Midwifery errors are associated with several problems for mother and fetus, which can lead to deadly consequences. On the other hand, if the causes of these errors are not identified, it is not...
Evaluation of Antibacterial Effect of Silver Nanoparticles, Ciprofloxacin, and their Combination against Pseudomonas aeruginosa PAO1
Background and Objectives: Pseudomonas aeruginosa is one of the most common causes of secondary infections. Considering the indiscriminate use of antibiotics and the risk of antibiotic resistance, studies on identificati...