Use of RAPD-PCR to reveal genetic proximity of Species of Chrysopidae Family (Insecta, Neuroptera)
Journal Title: International Journal of Farming and Allied Sciences - Year 2014, Vol 3, Issue 4
Abstract
RAPD (random amplification of polymorphic DNA) was used for distinguishing the genetic diversity between nine species of Chrysopidae family of Kermanshah province. Many specimens were collected in different locations of Kermanshah province, between them 27 specimens were identified to be of 9 species of Chrysopidae. The wing venation, male genitalia and other morphological characters were used for identification of the species belonging to Chrysopidae family and the determination of species were done by study of male genitalia. The extraction of DNA was done by use of CTAB method. Nineteen primers were used, among them thirteen primers have produced too many polymorph bands. Hundred thirty three bands were obtained by use of primers, among them hundred twenty six bands were polymorph and the rest monomorph. Total polymorphism was 94.7%, and size of the bands had a range of 300-350 bp. The maximum of polymorphism(100%) was obtained using OPA1, OPA7, OPA10, PKB b, PKBc, PKBd, OPH1, OPH5, OPH12,OPH20, POPFe, N7, N13 primers and the minimum of polymorphism (82.86%) was obtained using OPA2, OPA3, OPA11, OPA18, POA20, OPBa primers.
Authors and Affiliations
Alinaghi Mirmoayedi, Sahar Marami, Kherolah Yari and Danial Kahrizi
Keywords
Related Articles
- EP ID EP32576
- DOI -
- Views 266
- Downloads 0
Evaluating the effective of barriers and restrictions on applying precision agriculture in sari’s rice field (Iran), from the view of agriculture jihad’s experts
An precision agriculture is a managerial strategy which examines on special local details for management on product’ s puts on the needed basis of each area, so that economical production increases and the environment...
A study of storage behavior of okra fruits (Abelmoschus esculuntus L. Moenth cv. Khnesri )
This study was conducted during 2009-2010 season to investigate the effect of some antioxidants on storage behavior of okra fruits (Abelmoschus esculuntus L. Moenth cv. Mahaly). Okra seeds were planted under plastic tu...
Agroecology and application in agriculture
Agroecology combines scientific inquiry with indigenous and community-based experimentation, emphasizing technology and innovations that are knowledge-intensive, low cost and readily adaptable by small and medium-scale...
Effects of Shilajit Extract on Improving Burn in Rats
The most important factor in healing wounds, especially, the wound of burn,is prevention of its infection. Shilajit is the natural compound derived from plants in the rocky mountainous. Researchs have shown it has inhi...
Living condition of forest dwellers and their relationship with forest in Zagros
In most forest areas the living of people depends on forest and this value is significant in villages. There is a strong connection between plant societies and rural dwellers. Therefore, regarding to this value based o...