Use of salidroside in a lipopolysaccharide-induced periventricular leukomalacia model
Journal Title: Experimental Biomedical Research - Year 2018, Vol 1, Issue 4
Abstract
Aim: Research into the different treatment methods based on the intrauterine lipopolysaccharide (LPS)-induced Periventricular leukomalacia (PVL) model, as one of the main causes of morbidity in preterm infants still continues to be relevant. The present study investigates the effect on PVL of salidroside obtained from Rhodiola Rosea (golden root, orpin rose), which is a plant with known for its medicinal qualities. Method: To develop an induced PVL model, a 500 microgram/kg dose of LPS (Escherichia coli, serotype 055:B5, Sigma) was applied to two pregnant rats intraperitoneally on day 18, day 19 and day 20 of gestation. One of the LP applied rats was given 25 mg/kg Salidroside (250 mg Rhodiola root extract capsules, which include 3 mg Salidroside) by oral gavage (LPS+Salidroside), and a physiological saline solution was given to the control group. After delivery, 10 offspring of the LPS-applied mother, nine offspring of the LPS+Salidrosideapplied mother and seven offspring of the control mother were sacrificed on postnatal Day 7 with ether anesthesia. The caspase enzyme located in apoptosis pathways of 10 percent neutral-buffered formalin fixed brain tissue was stained immunohistochemically, and apoptotic cells were counted. Results: No statistically significant difference was noted between the LPS+Salidroside group and the control group, while a statistically significant difference was noted between the LPS and LPS+Salidroside groups. It was observed that Salidroside reduced LPS induced apoptosis. Conclusion: The intended experimental neuroprotective effect of Salidroside usage was provided through the inhibition of apoptosis in a PVL-damaged brain.
Authors and Affiliations
Mustafa Dilek, Gokce Kaya Dincel, Ayhan Cetinkaya, Mervan Bekdas, Nimet Kabakus
Keywords
Related Articles
- EP ID EP50815
- DOI https://doi.org/10.30714/j-ebr.2018443417
- Views 238
- Downloads 0
Effects of gestational exercise on hyperoxia-induced brain damage in the newborn
Aim: Preterm infants encounter hyperoxia relatively early on as they leave the intrauterine environment earlier than expected, while also being exposed to a higher level of hyperoxic stress due to insufficiencies in thei...
Is there a relationship between polycystic ovary syndrome and the FABP1 gene rs2197076 single nucleotide polymorphism?
Aim: Polycystic ovary syndrome (PCOS) is a multifactorial, endocrine, and metabolic disorder seen in 10%-20% of women of reproductive age. Due to the close relationship observed between the increased risk of type 2 diabe...
Use of salidroside in a lipopolysaccharide-induced periventricular leukomalacia model
Aim: Research into the different treatment methods based on the intrauterine lipopolysaccharide (LPS)-induced Periventricular leukomalacia (PVL) model, as one of the main causes of morbidity in preterm infants still cont...
Comparison of platelet volume indices in acute coronary syndrome
Aim: To compare the parameters showing the platelet volume index in unstable angina pectoris (USAP), non-ST elevation myocardial infarction (non-STEMI) and ST elevation myocardial infarction (STEMI). Methods: The plat...
The role of performing a routine four-quadrant cervical biopsy in patients with negative colposcopic findings in increasing the identification rate of cervical intraepithelial neoplasms
Aim: In patients with high-risk human papilloma virus (HPV), there is no consensus on the inclusion of cervical biopsy for diagnostic purposes in cases whereas there is no pathological finding in colposcopy. In this stud...