Usefulness of Kato-Katz and trichrome staining as diagnostic methods for parasitic infections in clinical laboratories

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Usefulness of Kato-Katz and trichrome staining as diagnostic methods for parasitic infections in clinical laboratories

Journal Title: Sultan Qaboos University Medical Journal - Year 2001, Vol 3, Issue 2

Abstract

Objectives: To assess the efficacy of the Kato-Katz technique and to re-evaluate other routine procedures conducted in the Microbiology Clinical Laboratory at Sultan Qaboos University Hospital (SQUH) and to throw light on the prevalence of intestinal parasitic infections among a small group of food handlers in Muscat. Method: Faecal samples collected from food handlers were examined using five parasitological techniques. Results: Out of ïœ±ïœ°ïœ° faecal samples, ïœµïœ³ were positive for one or more of ïœ±ïœ± intestinal parasites. The KatoKatz and trichrome stain methods were found superior to the other techniques in detecting helminthic and protozoan infections, respectively. The auramine stain was useful only in detecting Cryptosporidium parvum oocysts. Conclusion: A combination of trichrome stain and Kato-Katz techniques for stool examination is sufficient and recommended for busy laboratories; auramine stain should be applied only to samples with suspected cryptosporidal infections.

Authors and Affiliations

Mohamed A Idris| Department of Microbiology and Immunology, College of Medicine, Sultan Qaboos University, P.O. Box: , Al-Khod , Muscat, Sultanate of Oman, Ayda M Al-Jabri| Department of Microbiology and Immunology, College of Medicine, Sultan Qaboos University, P.O. Box: , Al-Khod , Muscat, Sultanate of Oman

Keywords

Kato-Katz wet preparation concentration trichrome and auramine stains intestinal parasites food handlers

EP ID EP13631
DOI -
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