USEFULNESS OF THE GRAPEVINE VIRUS-INFECTED COLLECTION
Journal Title: Current Trends in Natural Sciences - Year 2012, Vol 1, Issue 1
Abstract
In order to use the virus-infected material as reference in various studies, a grapevine virus collection was established at NRDIBH Ştefănşti-Argeş. The vines are infected with 1-3 of the main specific viruses of this crop: fanleaf virus, leafroll associated virus serotypes 1+3, fleck virus and virus A. Different lots of plants belonging to the same cultivar are infected with different viruses. The own rooted or grafted potted plants are maintained in an insect-proof greenhouse. The main goals of the study of grapevine under the influence of virus infection had in view: symptoms, in vitro behaviour of virus infected grapevine, virus elimination, plant positive control in the diagnostic process. The symptoms produced by viral infection can affect the whole plant (systemic symptoms) or they are visible on certain parts of the plant (local symptoms). In vitro studies of virus infected grapevines comparatively with the healthy material aimed with the quantitative and qualitative aspects of the culture: multiplication and rooting rates, shoots elongation, abnormal cuttings and vitrification phenomena. Infected grapevine cultivars and clones were subjected to virus elimination through thermotherapy, chemotherapy or electrotherapy, combined with in vitro culture. The diagnosis of leafroll, fleck, vein necrosis and corky bark diseases have been done by in vitro micrografting, as rapid biological method of virus detection. Samples collected from infected vines were used as material testing for virus detection by ELISA in inter-laboratory comparisons and Iaboratory-performed validation.
Authors and Affiliations
Elena-Cocuta Buciumeanu, Ionela Catalina Guta
Keywords
Related Articles
- EP ID EP24901
- DOI -
- Views 400
- Downloads 20
THE INCIDENCE OF MYCOBACTERIUM TUBERCULOSIS INFECTIONS AND RESISTANCE TO ANTITUBERCULOSIS DRUGS IN GIURGIU POPULATION
Tuberculosis due to Mycobacterium tuberculosis is an important health problem all over the world. Correct and early diagnosis of infection is a major condition for efficient treatment. Different localizations of mycobac...
THE SPECIFIC STRUCTURE, FUNCTIONAL ROLE AND ORGANISATION OF LUMBRICIDAE IN A SECONDARY NATURAL GRASSLAND
The present work analyses the indices of aggregation, dispersion, expansion, dominance and receptivity of lumbricidae fauna in a grassland located in the high hills of Piedmont Candesti in the south-east of Arges County...
THE USE OF KINETIN FOR THE EFFICIENT IN VITRO INITIATION OF SOME CULTIVARS OF STRAWBERRY
Strawberry is one of the first horticultural species introduced in the plant tissue culture technology. Nowadays microprogation technology is one of the most important techniques to produce plants with high quality and...
ECOLOGICAL RESEARCH ON AVIFAUNA FROM THE SITE NATURE 2000 ROSPA0062 – “LACURILE DE ACUMULARE DE PE ARGEŞ”. THE SEROTINAL SEASON
The present paper provides the results of the ecological research conducted on the avifauna of the reservoirs located in the middle valley of the Arges River during the serotinal season. The serotinal season was one of...
NEW CONTRIBUTIONS TO THE KNOWLEDGE OF THE BEETLES FAUNA OF PLUM ORCHARDS
Observations were made in 2015 in a plum fruit tree orchards of Pietrărie stationary from Bârnova belonging Iasi County. For the collection were used the soil traps Barber, the wet type. Six traps have been used in whic...