UTILITY OF AGAROSE CELL BLOCK TECHNIQUE FOR FINE NEEDLE ASPIRATION, URINE AND SEROUS EFFUSIONS CYTOLOGY, IN GEZIRA STATE.
Journal Title: World Journal of Pharmaceutical and Medical Research - Year 2017, Vol 3, Issue 1
Abstract
Objective: To assess utility of the agarose cell block technique based on specimen adequacy and diagnostic accuracy. Material and Methods: 63 study subjects were selected for this study, among them 12(19%) were males and 51(81%) were females with age range between 4-77 years and the mean age was 36.97 ± 1.9. One cell block section and one smear were made from each case, the study cases were divided into 4 groups according to the type of specimens including thyroid 23 (36.5%), breast 15(23.8%), lymph nodes 6 (9.5%) and soft tissue masses 9 (14.28). The rest include three (4.76 %) effusions (Two Ascetic fluids and One pleural fluid), and 7(11.11%) urine samples. The soft tissue masses include face lipoma, post auricular, arm, back, right mandible, foot, ankle, neck, and abdominal masses. Result: When comparing the final diagnosis of cell block sections and cytological smears according to the background, cellularity, the nuclear and cytoplasm preservation, 21(33.3%) of samples were better by cell block technique, 27(42.9%) showed same results in both cellblock technique and smearing technique and 15(23.8%) were found to be less using cell block technique. Conclusion: The study concludes that different cellblock sections should be used for, histochemical, immunohistochemical (IHC), and Immunocytochemical (ICC) investigations in diagnostic cytology laboratories. Molecular studies such as, fluorescent /chromogenic in-situ hybridization (FISH/CISH) and in-situ PCR, could also be applied on cell block sections.
Authors and Affiliations
Awad A Eltyeb
Keywords
Related Articles
- EP ID EP651138
- DOI -
- Views 222
- Downloads 0
RADIOLOGICAL ESTIMATION OF SEX USING TROCHLEAR SURFACE OF TALUS IN ADULT NORTH EASTERN NIGERIANS
Aim: To evaluate sexual differences using demarking points and index of sexual dimorphism using Trochlear Breadth of the talus in relation to age of males and females in north eastern Nigerians. Methodology: Three hundre...
MODELLING ISOLATED SPINA BIFIDA SCREENING PERFORMANCE USING AXIAL AND SAGITTAL VIEWS OF THE BRAIN AND SPINE ANATOMY AT THE 11-13-WEEK SCAN
Objectives: The aim of this research is to evaluate the modeled predictive value of different features of the fetal anatomy in the detection of isolated open spina bifida, at first-trimester scan. Methods: We retrospecti...
ANTIBACTERIAL PROPERTIES OF METHANOL AND AQUEOUS EXTRACTS OF ANOGEISSUS LEIOCARPUS AND TERMINALIA MICROPTERA AGAINST SELECTED ORAL PATHOGENS
In Africa, chewing sticks are commonly used for maintaining oral hygiene. Phytochemical contents and antibacterial activity of aqueous and methanol extracts of Terminalia microptera and Anogeissus leiocarpus were investi...
ALCOHOLIC EXTRACT OF ALOE VERA AS ANTIBACTERIAL AGENT AGAINST THE GRAM-POSITIVE BACTERIA STAPHYLOCOCCUS AUREUS IN MEDANI CITY - GEZIRA STATE – SUDAN - 2018
The plant Aloe vera was used historically as a topical to heal wounds, various skin conditions and orally as a laxative. The Gram-positive bacterium Staphylococcus aureus is considered to be the most pathogenic species o...
INFLAMMATION, HORMONE IMBALANCE: A PREDISPOSING FACTORS OF BENIGN PROSTATIC HYPERPLASIA: GENERAL REVIEW
Benign prostatic hyperplasia (BPH) continues to be a significant healthcare problem world-wide. Recently published data suggest that clinical BPH, which is hallmarked by the occurrence of moderate-to-severe lower urinary...