UTILITY OF LINE PROBE ASSAY FOR IDENTIFICATION OF MDR-TB AND NTM IN SMEAR POSITIVE SPUTUM SAMPLES FROM A TERTIARY CARE HOSPITAL OF WESTERN U.P. INDIA.
Journal Title: Indian Journal of Scientific Research - Year 2017, Vol 8, Issue 1
Abstract
Detection of the MDR & XDR status and NTM identification from smear positive sputum samples by Line Probe Assay Study was performed on smear positive sputum samples collected from DOTS centre at TMMC & RC, Moradabad during period of July 2015 to March 2017. Samples were decontaminated by Pettrof method and then DNA was extracted by using heating method, and then were subjected to PCR using Applied Biosystem Veriti 96 well Thermal Cycler and Line Probe Assay by using GenoType MTBDRplus on Twincubater of HAIN life sciences. Total 200 smear positive sputum samples were processed by line probe assay, in which 16 were MDR, 2 were XDR and 10 were detected as NTM. In this study, we concluded that, on routine basis we should use line probe assay for drug sensitivity of M.tuberculosis cases, so that appropriate treatment can be given on time to the patients.
Authors and Affiliations
SANA NUDRAT
Keywords
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