Utility of Modified Ultrafast Papanicolaou Stain in Cytological Diagnosis
Journal Title: Journal of Clinical and Diagnostic Research - Year 2017, Vol 11, Issue 3
Abstract
Introduction: Need for minimal turnaround time for assessing Fine Needle Aspiration Cytology (FNAC) has encouraged innovations in staining techniques that require lesser staining time with unequivocal cell morphology. The standard protocol for conventional Papanicolaou (PAP) stain requires about 40 minutes. To overcome this, Ultrafast Papanicolaou (UFP) stain was introduced which reduces staining time to 90 seconds and also enhances the quality. However, reagents required for this were not easily available hence, Modified Ultrafast Papanicolaou (MUFP) stain was introduced subsequently. Aim: To assess the efficacy of MUFP staining by comparing the quality of MUFP stain with conventional PAP stain. Materials and Methods: FNAC procedure was performed by using 10 ml disposable syringe and 22-23 G needle. Total 131 FNAC cases were studied which were lymph node (30), thyroid (38), breast (22), skin and soft tissue (24), salivary gland (11) and visceral organs (6). Two smears were prepared and stained by MUFP and conventional PAP stain. Scores were given on four parameters: background of smears, overall staining pattern, cell morphology and nuclear staining. Quality Index (QI) was calculated from ratio of total score achieved to maximum score possible. Statistical analysis using chi square test was applied to each of the four parameters before obtaining the QI in both stains. Students t-test was applied to evaluate the efficacy of MUFP in comparison with conventional PAP stain. Results: The QI of MUFP for thyroid, breast, lymph node, skin and soft tissue, salivary gland and visceral organs was 0.89, 0.85, 0.89, 0.83, 0.92, and 0.78 respectively. Compared to conventional PAP stain QI of MUFP smears was better in all except visceral organ cases and was statistically significant. MUFP showed clear red blood cell background, transparent cytoplasm and crisp nuclear features. Conclusion: MUFP is fast, reliable and can be done with locally available reagents with unequivocal morphology which is the need of the hour for a cytopathology set-up.
Authors and Affiliations
Prachi Sinkar
Keywords
Related Articles
- EP ID EP394009
- DOI 10.7860/JCDR/2017/20882.9532
- Views 49
- Downloads 0
Renal Failure: The Most Important Prognostic Factor for Morbidity and Mortality in Patients Undergoing Open Elective Abdominal Aortic Aneurysm Repair
ABSTRACT Introduction: The elective abdominal aortic aneurysm repair leads to a remarkable decrease in the rupture of aneurysm and an increase in the life expectancy among patients. Aim: To study the mortality rate and c...
Immune Response in Pregnant Women Infected with Acute Vaginal Abscess Caused by Staphylococcus aureus and Trichomonas vaginalis
ABSTRACT Introduction: Vaginal abscess in pregnant women caused by Staphylococcus aureus and Trichomonas vaginalis is one of the most important disease. This infection is still medically uncontrolled because of poor heal...
Brugada Syndrome with Spontaneous Fluctuation in ECG Pattern
Images in Medicine
Malignant Granular Cell Tumour Presenting as a Paravertebral Mass in an Adolescent Male- A Rare Presentation of an Uncommon Tumour
Granular Cell Tumour (GCT), also known as Abrikossoff’s tumour is a rare neural tumour, mostly benign and solitary but rare malignant and multifocal occurrence are also reported. Location of tumour varies widely within b...
Effect of Green Coffee Bean Extract on Streptococcus mutans Count: A Randomised Control Trial
Introduction: Mouth rinses have been popularly used as a supplementary oral hygiene aid. A lot of commercially available mouth rinses possess few adverse effects, which has necessitated the search for alternative and her...