Utility of Xpert MTB/RIF assay for diagnosis of childhood tuberculosisExtrapulmonary and Pulmonary
Journal Title: Journal of Medical Science And clinical Research - Year 2018, Vol 6, Issue 5
Abstract
Background: Xpert MTB/RIF assay is now recommended by WHO for diagnosis of tuberculosis in children but evaluation data is limited. Methods: The study was conducted on 186 consecutive specimens (both respiratory and non respiratory) collected between October 2013 to July 2016 from 171 children having MDR TB or/and clinically suspected tuberculosis. Of the 186 specimens, maximum were sputum 83 (44.6%) followed by gastric aspirate 46 (24.7%), CSF 34 (18.3%), tissue biopsies 8 (4.3%), pleural fluid 6 (3.2%), ascitic fluid 5 (2.6%) and lymph node aspirates 4 (2.2%). Each specimen was subjected to smear examination (direct and after decontamination), culture on Lowenstein Jensen (LJ) medium and Xpert MTB/RIF assay. Results: Analyses on per-sample basis showed that direct smear was positive in 54 (29.2%), culture in 78 (42.2%) and Xpert MTB/RIF assay in 77 (41.6%) specimens. Overall sensitivity and specificity of Xpert MTB/RIF was 93.6% and 96.2% while that of smear was 69.7% and 98.9% respectively against culture as the gold standard. Of the total 77 specimens positive by Xpert MTB/RIF, rifampicin resistance was observed in 7 (9.1%). Conclusion: Xpert MTB/RIF assay is a promising tool for diagnosis as well as detection of rifampicin resistance because of its high sensitivity, specificity and rapidity. However, as more than 50% of clinically suspected children still remain unconfirmed, further refinement of the test before it can replace the current reference standards for diagnosis is to be looked into.
Authors and Affiliations
Dr Shilpa Arora
Keywords
Related Articles
- EP ID EP513299
- DOI -
- Views 61
- Downloads 0
Effect of Antihypertensive Drugs on Glucose Tolerance and Lipid Profile in Hypertensives With and Without Diabetes Mellitus
Diabetes is the most common endocrine disease. It is a heterogeneous primary disorder of carbohydrate metabolism with multiple etiologic factors that generally involve absolute or relative insulin deficiency or insulin r...
A Rare Case of Double Jejunal Transection after Blunt Abdominal Trauma
The blunt abdominal trauma have become increasingly common in this era of speed and hurry. The machines now occupied position of master of man rather than man who was the master of machine. The speed , unwanted hurry, sh...
Alpha-1-Antitrypsin Activity in Bronchiectasis
Bronchiectasis is an uncommon disease with the potential to cause devastating illness.1 Although fewer than 100 cases of bronchiectasis associated with alpha-1-antitrypsin deficiency have been reported there is little do...
A Study of Branching Pattern and Distribution of Coronary Arteries in Adult Human Heart
Heart receives its blood supply from the coronary vessels. This current study is done to throw light upon the number of coronary ostia, diameter of the trunk, arterial supply to S A node and A V node, termination of righ...
Study on recent trends in antibiogram of enteric fever pathogens in a tertiary care centre in South India
Enteric fever is a systemic infection caused by Salmonella Typhi (S.Typhi), Salmonella Paratyphi A (S.Paratyphi A), Salmonella Paratyphi B and Salmonella Paratyphi C. It is endemic in India and is a major public health p...