UV SPECTROPHOTOMETRIC AND RP- HPLC METHODS FOR SIMULTANEOUS ESTIMATION OF ISONIAZID, RIFAMPICIN AND PIPERINE IN PHARMACEUTICAL DOSAGE FORM
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 10
Abstract
Objective: Simple, reliable, sensitive and accurate spectrophotometric and RP-HPLC methods for the estimation of INH, RIFA and PIPE in pure and pharmaceutical dosage form.Methods: In the first Absorption correction method, methanol and distilled water were used as diluent. The wavelengths selected for the analysis were 262 nm, 338 nm and 477 nm for INH, PIPE and RIFA respectively. The Second RP – HPLC method has been developed using Acetonitrile as diluent. Successful separation of drugs was achieved on LC18 100 Aâ° column (250 x 4.6 mm, 5 μ) using 0.01M Sodium Dihydrogen Orthophosphate, pH 6.5 and acetonitrile (40:60, % v/v) as mobile phase with flow rate of 0.9 mL/min. The wavelength of detection was 282 nm. Validation of developed methods was done according to ICH Q2 (R1) guideline.Results: Calibration curve was linear over the concentration range of 12-34.5 μg/mL (INH), 8-23 μg/mL (RIFA) and 0.4-1.15 μg/mL (PIPE) respectively for absorption correction method and 30- 330 μg/mL (INH), 20-220 μg/mL (RIFA) and 1-11 μg/mL (PIPE) for RP – HPLC method with r2 value greater than 0.995. Accuracy of methods was determined by recovery studies and it was found to be 98 to 102 %. The % RSD values for all the validation parameters were less than 2.0 % for both the methods.Conclusion: The developed RP-HPLC and UV spectrophotometric method were successfully applied for the quantitative determination of cited drugs in pharmaceutical dosage form.Â
Authors and Affiliations
Umang H Shah, Abkita H Jasani
Keywords
Related Articles
- EP ID EP579448
- DOI -
- Views 73
- Downloads 0
BIOACTIVITIES OF THE THAI MEDICINAL AND EDIBLE PLANTS C. CAJAN, M. CITRIFOLIA AND O. AMERICANUM
Objective: Inflammation and oxidative stress are closely related and play a role in various diseases. If an infectious component plays a role, an antibacterial effect is of advantage. Thus, natural remedies which combine...
ANTIMICROBIAL RESISTANCE OF PSEUDOMONAS AERUGINOSA STRAINS FROM PATIENTS WITH URINARY TRACT INFECTIONS IN SBMPMC HOSPITAL BIJAPUR, INDIA
Objectives: Urinary tract infections (UTIs) are one of the most common bacterial infections affecting humans throughout their life span. There is paucity of literature in relation to pathogenesis of UTIs caused by Pseudo...
ANAESTHETIC MANAGEMENT OF A PATIENT WITH PREVIOUS LOBECTOMY POSTED FOR EMERGENCY MODIFIED RADICAL MASTOIDECTOMY
Pulmonary disease can be considered as a risk factor for several respiratory complications occurring during the perioperative period. Here we present a case of a middle-aged man who underwent modified radical mastoidecto...
MEMECYLON SPECIES: A REVIEW OF TRADITIONAL INFORMATION AND TAXONOMIC DESCRIPTION
The present review is to avail the comprehensive information on taxonomy, phytochemistry and pharmacology of Indian Memecylon species. Memecylon is one of the complex genus of flowering plants and it is an important sour...
LINSEED OIL SUPPLEMENTATION IMPROVES LECITHIN CHOLESTEROL ACYLTRANSFERASE AND TISSUE LIPASE ACTIVITIES IN PREGNANT OBESE RATS AND THEIR OFFSPRING.
Objective: The aim of the present work was to study how maternal dietary linseed oil modulates lecithin cholesterol acyltransferase (LCAT) and adipose tissue lipase responses to cafeteria diet in rats during pregnancy an...