UV SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION FOR ESTIMATION OF SITAGLIPTIN PHOSPHATE IN BULK AND TABLET DOSAGE FORM BY ABSORPTION RATIO AND AREA UNDER THE CURVE METHOD
Journal Title: Journal of Biological and Scientific Opinion - Year 2013, Vol 1, Issue 4
Abstract
To develop two simple UV spectrophotometric methods for simultaneous estimation of Sitagliptin phosphate (STG) in bulk and tablet dosage forms and validates as per ICH guidelines. Method A involved Absorbance maxima method which based on the measurement of absorbance at λ max of Sitagliptin phosphate 267 nm and Method B involved Area under the curve (AUC) method which based on the measurement of AUC in the range of 261-270 nm. The developed methods were validated for linearity, precision, accuracy, LOD and LOQ as per ICH guidelines. Both the methods were found to be linear within the conc. range of 20-160 µg/ml for Sitagliptin phosphate. The present methods were found to be simple, linear, precise, accurate and sensitive and can be used for routine quality control analysis for the estimation of Sitagliptin phosphate in bulk and tablet dosage form.
Authors and Affiliations
Vinit Chavhan, Atul Kadam
Keywords
Related Articles
- EP ID EP99134
- DOI 10.7897/2321–6328.01407
- Views 140
- Downloads 0
A NEW SPECIES EUDORINA EHRENBERG (VOLVOCACEAE, CHLOROPHYTA) FROM BULGARIA
From a small ephemeral basin in Plovdiv (Bulgaria) were found and described a new species of the genus Eudorina (Chlorophyta, Volvcoales) - E. plurivacuolata sp. nov. The closest to the newly described species are Eudori...
TRAYOPASTHAMBAS: THREE SUPPORTIVE PILLARS OF AYURVEDA
The Ayurvedic classical text Charka Samhita describes an analogous between the human body and a building. Any building requires pillars for its stability. The Sanskrit term for a pillar is Sthambha. Ayurveda state that f...
CRANIAL FACIITIS: A RARE VARIANT IN CHILDHOOD
Cranial fasciitis is a rare variant of nodular fasciitis, which is seen exclusively in infants and childhood, mainly in the head and neck region. It is considered as non-neoplastic lesion similar to nodular fasciitis. A...
AYURVEDIC MANAGEMENT OF SWITRA WITH SPECIAL REFERENCE TO VITILIGO: A CASE REPORT
Skin is the best indicator of general health. According to Ayurveda skin is one of the essential sense organ. As skin covers the whole body, bhrajaka pitta should be maintained in a proper state, an imbalance in vata and...
A CROSS SECTIONAL STUDY ON DENTAL FLUOROSIS AMONG SCHOOL CHILDREN INCLUDING CHEMICAL ANALYSIS OF POTABLE WATER SOURCES IN ADPALLI PHC, MULCHERA, GADCHIROLI, MAHARASHTRA, INDIA
Dental fluorosis is a developmental disturbance of dental enamel, caused by successive exposures to high concentrations of fluoride during tooth development, leading to enamel with lower mineral content and increased por...