U.V Visible Spectrophotometric Determination, Validation and Invitro Interaction of Rabeprazole with Chlorazepate
Journal Title: Saudi Journal of Medical and Pharmaceutical Sciences - Year 2017, Vol 3, Issue 8
Abstract
Abstract: In the present work a simple, rapid, accurate and precise method has been developed and validated for the determination of Rabeprazole in bulk using UV/visible spectroscopy. Rabeprazole is used for the treatment of GERD and duodenal ulcer. In this development methanol is used as a solvent and solution was scanned at 200 to 400nm range, rabeprazole showed maximum absorbance at 284nm. Linearity was observed in the concentration range of 12-18ug/ml. Percent recovery was found from 99.86% - 100.14%, %RSD for repeatability was calculated 0.628% and for precision inter and intraday was found from0.488% - 0.77% representing the accuracy, repeatability and precision of the method. Validation of the method was carried out for its linearity, accuracy, precision, specificity according to ICH guidelines. On the basis of results obtained it is concluded that the proposed method can be used for the routine analysis of rabeprazole. After method validation interaction studies were performed with chlorazepate dipotassium, both were taken in 1:1 ratio and readings were observed on UV/Visible spectrophotometer for 3 hours at buffers 4, 7.4 and 9. Results support to the fact that rabeprazole interact with chlorazepate dipotassium. Keywords: rabeprazole, spectrophotometric determination, validation, interaction, chlorazepate
Authors and Affiliations
Somia Gul, Sania Basheer
Keywords
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