VALIDATED HPTLC METHOD OF ANALYSIS OF NIMESULIDE IN BLOOD AND TISSUE DISTRIBUTION IN DIFFERENT ORGANS OF RATS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2012, Vol 3, Issue 10
Abstract
Nimesulide, (4-nitro-2-phenoxymethane-sulfoanilide), a NSAID compound has been used in human and veterinary medicine having a selective cyclooxygenase-2 (COX-2) inhibition. Besides its potential anti-inflammatory effect, it has been reported that long term administration of nimesulide caused hepatotoxicity in human being. In the present study in order to determine the tissue distribution of nimesulide, the albino rats were administered at a dose rate of 4.5mg/kg body wt. once daily at regular interval through oral route for five days. After five days of dosing blood and tissue samples were collected at predetermined intervals (6,12, 24 and 48hrs). A more sensitive and validated HPTLC analysis method was developed to determine the concentration of this drug in plasma and various tissues such as liver, kidneys, lungs, heart, spleen, and brain. The tissue concentration of nimesulide at 6,12,24 and 48hrs post-administration were compared with plasma concentration. Nimesulide concentration was estimated to be the highest in kidneys at 6,12,24 and 48hrs post administration amongst all tissues examined. The rank order AUC (tissues)/AUC (plasma) ratio was highest for kidney (1.73±0.4) followed by brain, spleen, heart, liver and lungs. The present results suggest that (i) nimesulide concentration during 48hr following withdrawal, declined in a faster rate in plasma and slower rate in tissues like liver,lungs, heart,spleen and brain which accounts about 10-18% of maximal plasma concentration (ii) in kidneys its concentration is maintained at 85% of maximum plasma concentration at 48hr foloowing withdrawal. In conclusion kidney retains a significantly higher concentration of nimesulide upto 2 days following withdrawal of five-day administartion in rats. It is well predicted from the observation obtained from the rat model that (i) kidney damage could be alarming in long term therapeutic administartion to animal and man and (ii) animals and birds could be unsafe consuming the dead animals administered with nimesulide recntly.
Authors and Affiliations
Lombodar Mahapatra , Gayana Sahoo , Monoj Panda , Subas Parija
Keywords
Related Articles
- EP ID EP156249
- DOI -
- Views 91
- Downloads 0
CLINICAL PRESENTATION AND ASSOCIATION AMONG TUBERCULOSIS PATIENTS; COHORT COMPARISON BETWEEN SMOKERS VERSUS NEVER-SMOKE IN PENANG, MALAYSIA
We aimed to compare the demographic and clinical characteristics with risk determination of TB patients who were smokers vs. non-smokers. The retrospective, observational & cross-sectional cohort survey was done to c...
EVALUATION OF ANTIUROLITHIATIC ACTIVITY OF ETHANOLIC EXTRACT OF ANNONA RETICULATA AGAINST ETHYLENE GLYCOL AND AMMONIUM CHLORIDE INDUCED UROLITHIASIS IN ALBINO WISTAR RATS
Urinary stone disorder has afflicted humankind since antiquity and can persist, with serious medical consequences, throughout a patient’s lifetime. The common component of urinary stone is calcium oxalate (CaOx). In spit...
RP-HPLC METHOD FOR THE SEPARATION OF PARACETAMOL, TIZANIDINE, ACECLOFENAC, CHLORZOXAZONE: APPLICATION TO TIZANIDINE DETERMINATION IN TABLET DOSAGE FORM
New, rapid, precise, accurate and specific chromatographic method with UV detection was developed and validated for the separation of paracetamol, tizanidine, diclofenac and chlorzoxazone and applied for the determinatio...
RecA: A NOVEL TARGET FOR DRUG DELIVERY
The RecA protein is a recombinase functioning in recombinational DNA repair in bacteria. RecA is regulated at many levels. The expression of the recA gene is regulated within the SOS response. The activity of the RecA pr...
SKIN KINETICS AND DERMAL CLEARANCE
Availability of several therapeutic and cosmetic formulations for topical application has made the research on skin kinetics as a topic of current interest. Topical formulations are typically meant for local effect altho...