Validated RP-HPLC Method for Quantification of Paclitaxel in Human Plasma – Eliminates Negative Influence of Cremophor El
Journal Title: International Journal of Current Research and Review - Year 2018, Vol 10, Issue 13
Abstract
Background: Literature reports innumerable methods for quantification of paclitaxel in biological matrices. Most of these involve complicated extraction procedures like solid phase extraction, separate procedure for elimination of interference of Cremophor El, advanced and expensive instruments. Objectives: The objective of the present research work is to develop and validate a simple, rapid, sensitive, economic and reproducible reverse phase – high performance liquid chromatography method for the estimation of paclitaxel concentration in human plasma that eliminates negative influence of Cremophor El on recovery of paclitaxel. Methods: Chromatographic separation of paclitaxel was carried out using C18 column (150 × 4.6 mm i.d., 4μm particle size, Waters, Australia) with 60% acetonitrile, 40% of 10mM ammonium acetate buffer solution and 0.1% formic acid as a mobile phase at a flow rate of 1.0mL/min at ambient temperature. Validation was performed as per ICH Q2 guidelines. Results: In this system the retention time was 3min. The detection limit was 5ng/mL and limit of quantification with reproducibility was 15ng/mL. Plasma samples were extracted using single solvent (tertiary – Butyl Methyl Ether) liquid- liquid extraction with a recovery of 96-99%. Robustness of the method was established with variation in flow rate, detection wave length and mobile phase composition. Stability of paclitaxel during the study period was studied and found to be stable. Conclusion: The developed method is easy to perform, quick, reproducible with good recovery without negative influence of Cremophor El and applicable to quantify paclitaxel in regular clinical practice for individualization of the therapies.
Authors and Affiliations
Mangathayaru K.
Keywords
Related Articles
- EP ID EP533379
- DOI 10.31782/IJCRR.2018.10132
- Views 133
- Downloads 0
A Prospective Study to Analyse Safety of Low Flow Anesthesia for Laparoscopic Procedures
Background: Anesthesia for laparoscopic surgery has developed and advanced significantly, resulting in a technique that is safe and provides better outcome than before. Low flow anesthesia is newer technique which is mor...
Stratosphere-mesosphere Coupling Through Vertically Propagating Gravity Waves During Mesospheric Temperature Inversion (MTI): An Evidence
Objective: It is theoretically observed that atmospheric gravity waves play a key role in vertical coupling during the Mesosphere Temperature Inversion (MTI). Therefore, the present paper describes the observational evid...
Knowledge and Attitude of Parents Regarding their Children’s Oral Health in Buraydah City of Kingdom of Saudi Arabia
Background: Parents can help their children to sustain health oral habits, provided they themselves have good knowledge about oral disease and methods of its prevention. Aim: To assess parent’s knowledge and attitude reg...
Axial length and Refractive Status of Adults in South Western Nigeria
Background: The axial length of the eye is the distance between the centre of the cornea and the retina. This is one of the determinants of the refractive state of the eye and a knowledge of this is useful in intraocular...
In Vitro Interaction Studies between Artemether – Lumefantrine and Lamivudine/Metronidazole
This work assesses tablets of artemether and lumefantrine in vitro drug interaction with lamivudine or metronidazole. Spectra changes on artemether or lumefantrine vibration bands were evaluated using Fourier transform i...