VALIDATED SPECTROFLUORIMETRIC METHOD FOR THE DETERMINATION OF DORZOLAMIDE HYDROCHLORIDE IN ITS PURE FORM AND PHARMACEUTICAL FORMULATIONS VIA DERIVATIZATION WITH 4-CHLORO-7-NITROBENZO-2-OXA-1, 3- DIAZOLE (NBD-CL)
Journal Title: Innoriginal: International Journal Of Sciences - Year 2018, Vol 5, Issue 4
Abstract
Objectives: Simple spectrofluorimetric method was applied and validated for the determination of dorzolamide hydrochloride in its pure form and pharmaceutical formulations. Method: Based on nucleophilic substitution reaction of dorzolamide with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazole (NBD-Cl) to form a highly fluorescent fluorophore measured at 541 nm after excitation at 404 nm. Results: Under optimum condition, the proposed method obeys Beer’s law in range (1- 8 μg/mL). Conclusion: The method was validated according to ICH guideline for accuracy, precision and was successfully applied for the determination of dorzolamide its pure form and pharmaceutical formulations. Statistical comparison of the obtained results with the other results of the reported method indicated the good agreement of the proposed method.
Authors and Affiliations
AHMED A. ALMRASY, RAGAB A. SAID, AHMAD A. ABO-SERIE, KHALID A. M. ATTIA, NASR M. EL-ABASAWY
Keywords
Related Articles
- EP ID EP550363
- DOI -
- Views 112
- Downloads 0
DEVELOPMENT AND OPTIMIZATION OF HYDRODYNAMICALLY BALANCED GASTRORETENTIVE FLOATING TABLETS OF ATENOLOL
The present work was aimed to develop a floating drug delivery system of Atenolol based on novel approaches and to investigate there In vitro drug release and buoyancy performance. The interaction between the excipcents...
PHARMACOGNOSTICAL AND PHYTOCHEMICAL STUDY OF ACACIA CATECHU (LINN. F.) WILLD. &AZADIRACHTA INDICA (A. JUSS.)
Objective: In the present study Acacia catechu (Linn.f.) Willd. And Azadirachta indica (A. Juss.) were chosen for Pharmacognostical study and also evaluated chemically. Method: Bark of Acacia catechu (Linn.f.) Willd. And...
EFFECT OF FK 506 ON SELECTED ENZYMATIC ACTIVITY LEVELS INVOLVED IN PROTEIN METABOLISM IN ALBINO RAT TISSUES
Background: The plant Gmelina arborea has been traditionally used in India for several medicinal purposes like anthelmintic, diuretic, anti-inflammatory, antibacterial and antidiabetic. Aims: The aim of the present study...
A COMPARATIVE CLINICAL EVALUATION OF ACACIA CATECHU (LINN. F.) WILLD. & AZADIRACHTA INDICA (A. JUSS.) W.S.R. TO DIABETES MELLITUS
Objectiv: Comparison of effectiveness of drug Khadira twak churna in Group A and Nimba twak churna in Group B given same dose on patients of Diabetes mellitus. Method: Patients between 30-70 yrs. of age with clinical si...
VALIDATED SPECTROFLUORIMETRIC METHOD FOR THE DETERMINATION OF DORZOLAMIDE HYDROCHLORIDE IN ITS PURE FORM AND PHARMACEUTICAL FORMULATIONS VIA DERIVATIZATION WITH 4-CHLORO-7-NITROBENZO-2-OXA-1, 3- DIAZOLE (NBD-CL)
Objectives: Simple spectrofluorimetric method was applied and validated for the determination of dorzolamide hydrochloride in its pure form and pharmaceutical formulations. Method: Based on nucleophilic substitution reac...