VALIDATED SPECTROFLUORIMETRIC METHOD FOR THE DETERMINATION OF DORZOLAMIDE HYDROCHLORIDE IN ITS PURE FORM AND PHARMACEUTICAL FORMULATIONS VIA DERIVATIZATION WITH 4-CHLORO-7-NITROBENZO-2-OXA-1, 3- DIAZOLE (NBD-CL)
Journal Title: Innoriginal: International Journal Of Sciences - Year 2018, Vol 5, Issue 4
Abstract
Objectives: Simple spectrofluorimetric method was applied and validated for the determination of dorzolamide hydrochloride in its pure form and pharmaceutical formulations. Method: Based on nucleophilic substitution reaction of dorzolamide with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazole (NBD-Cl) to form a highly fluorescent fluorophore measured at 541 nm after excitation at 404 nm. Results: Under optimum condition, the proposed method obeys Beer’s law in range (1- 8 μg/mL). Conclusion: The method was validated according to ICH guideline for accuracy, precision and was successfully applied for the determination of dorzolamide its pure form and pharmaceutical formulations. Statistical comparison of the obtained results with the other results of the reported method indicated the good agreement of the proposed method.
Authors and Affiliations
AHMED A. ALMRASY, RAGAB A. SAID, AHMAD A. ABO-SERIE, KHALID A. M. ATTIA, NASR M. EL-ABASAWY
Keywords
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