Validated UV Spectrophotometric Methods for the Estimation of Olanzapine in Bulk, Pharmaceutical Formulations and Preformulation Studies
Journal Title: Journal of Pharmaceutical Research International - Year 2015, Vol 6, Issue 3
Abstract
Aims: To develop and validate two accurate and precise UV spectrophotometric methods for the estimation of widely prescribed anti psychotic drug-Olanzapine in bulk, formulations and preformulation studies. Methodology: Two different solvent systems optimized for the analysis (100 mM hydrochloric acid with pH 1.2 and phosphate buffer saline with pH 7.4), were based on various criteria such as applicability of the method, sensitivity, ease of sample preparation, cost effectiveness, stability of drug in investigating medium, and reproducibility. Olanzapine was estimated at 258 nm in 100 mM hydrochloric acid and 252 nm in phosphate buffer saline. Results: The linear regression equations obtained by applying least square regression analysis for Olanzapine were found to be: Absorbance = 0.0697 × concentration in µgml–1+ 0.008; r2= 0.9999) in 100 mM hydrochloric acid; absorbance = 0.0571 × concentration in µgml–1- 0.0052; r2 = 0.9998) in the phosphate buffer saline. Sandell’s sensitivity was found to be about 0.0144 µgcm−1 for hydrochloric acid and 0.0175 µgcm−1 for phosphate buffer saline. The apparent molar absorptivity was calculated to be 2.17x104 mol−1cm−1 and 1.78x104 mol−1cm−1 in hydrochloric acid and phosphate buffer saline respectively. Conclusion: Two spectrophometric methods for the analysis of Olanzapine have been developed successfully and validated as per ICH guidelines. Developed methods were successfully applied for the determination of Olanzapine in commercial tablets of varying strengths and various preformulation parameters of the drug such as dissociation constant.
Authors and Affiliations
Emil Joseph, Garima Balwani, Vibhu Nagpal, Satish Reddi, Ranendra Narayan Saha
Keywords
Related Articles
- EP ID EP344271
- DOI 10.9734/BJPR/2015/16361
- Views 98
- Downloads 0
Supernumerary Nostril in the Twin: Case Report
Supernumerary nostril is a rare congenital nasal deformity that contains additional nostril. We encountered a patient with isolated supernumerary nostril and presented our findings and surgical technique.
Evaluation of the Physicochemical and in vitro Dissolution Properties of Metformin Hydrochloride Tablet Brands Marketed in Five Cities in Ghana
Aim: A comparative evaluation of the physicochemical and in vitro dissolution properties of metformin hydrochloride tablet brands sampled in five cities in Ghana was undertaken to assess their pharmaceutical equivalence....
Isolation and Phytochemical Characterization of Bioactive Constituents from the Seeds of Garcinia kola, Heckel (Clusiaceae)
Aim: This study was carried out with the aim of isolating and identifying the bioactive constituents of the ethyl acetate extract from G. kola seeds that was a strong inhibitor of α-glucosidase using FT-IR and GC-MS tech...
Hepatoprotective Activity of Macrotyloma uniflorum (Lam.) Verdc. Seeds Extracts Against Paracetamol Induced Hepatotoxicity in Wistar Albino Rats
Background: Macrotyloma uniflorum (Horse gram) seeds are used in Ayurvedic medicine for treating numerous health disorders including rheumatism, worm, conjunctivitis and piles. The seeds were boiled with water and used a...
Melatonin Improves Metabolic Abnormalities Induced by HAART in Mice
Aims: Highly Active Antiretroviral Therapy (HAART) is associated with metabolic complications. An unexpected extension of functional implications of melatonin, the neuro-hormone synthetized during the night, has been rep...