Validation of CK-19 and HBME-1 Expression Pattern in FNACs to Supplement Morphological Evaluation for Preoperative Diagnosis of Thyroid Lesions
Journal Title: Journal of Advances in Medicine and Medical Research - Year 2016, Vol 13, Issue 4
Abstract
Aim: Fine Needle Aspiration Cytology is the cost effective, quick and minimally invasive method for the initial evaluation of thyroid nodule. But it poses a diagnostic challenge in differentiating benign follicular adenoma from follicular carcinoma and follicular variant of papillary carcinoma as they have similar cytological appearance. Present study focused on the identification of a biological marker for the differential diagnosis of thyroid malignancy in indeterminate cases. Place and Duration of the Study: Division of cancer Research, Regional Cancer Centre, Kerala, India, between August 2009 to September 2014. Methodology: Immunohistochemistry was performed using Ret (Rearranged during transfection), Hector Battifora Mesothelial Cell Antigen-1 (HBME-1), Cytokeratin-19 (CK-19), Keratan sulphate (KS), Thyroid peroxidase (TPO), Estrogen receptor (ER) and Progesterone receptor (PR) on cell block prepared from FNA material and corresponding tissue sections on 153 samples. Western blot analysis of ER and PR was performed in surgically excised fresh tissue specimens. Results: The present study found that HBME-1 is highly significant (P < .001) for the differential diagnosis with a diagnostic accuracy of 95.96%. Ret immunostaining may serve as good indicator of PTC whereas its sensitivity is very low in other lesions. The diagnostic accuracy of CK-19 was 90.91%. Intense positive staining of TPO was noted in majority of follicular epithelial cells of benign lesions (42.42%). Females had an increased prevalence in our study population so we examined the estrogen and progesterone receptors status in thyroid lesions. The specificity of KS, ER and PR are very low for the differentiation of adenoma from carcinomas of the thyroid. Conclusion: Present study found that the combination of two markers may give a more accurate way in the differentiation of thyroid nodules in problematic cases. So we suggest morphological evaluation along with immunocytochemistry of HBME-1 and CK19 can help the differential diagnosis of thyroid lesions in FNAs.
Authors and Affiliations
Shanone C. Pereira, V. T. Jissa, Jestin K. George, Shaji Thomas, K. Sujathan
Keywords
Related Articles
- EP ID EP340620
- DOI 10.9734/BJMMR/2016/23061
- Views 47
- Downloads 0
Assessing the Influence of Exposure to Violent Living Condition, Knowledge, and Personal Motivation on Human Immunodeficiency Virus Infection Prevention Behavior among Historically Black College Students
Aim: This study assesses the effects of prevention education, prevention personal motivation, prevention knowledge, and past exposure to violent living conditions on human immunodeficiency virus (HIV) infection preventio...
The Use of Magnification and Work Posture in Dentistry – A Literature Review
Background: Loupes represent a type of magnification and their use can promote higher quality on dental treatment. Besides those advantages magnification can be beneficial for musculoskeletal health maintenance on dental...
An Unpleasant Result of Imprecision: Esophageal Corrosive Injury Due to the Oral Intake of 10% Benzalkonium Chloride
Benzalkonium chloride is a skin antiseptic agent. It should be diluted before usage for cleaning of mucosal membranes; otherwise it may result in severe damage on mucosa. Herein we will report a 2 months old baby who too...
Exploring the Role of Spirituality in Coping Process of Family Caregivers of Patients in Vegetative State
Introduction: The vegetative state (VS) is a condition which can occurs after a severe brain injury. After hospital discharging Responsibility of caring of patients in vs is transferred to their families, which cause hig...
Patient Satisfaction with Services at a General Outpatient Clinic of a Tertiary Hospital in Nigeria
Aims: To determine the factors influencing patient satisfaction with services at the general outpatient clinic of a tertiary hospital in Nigeria. Study Design: A descriptive cross sectional study. Place and Duration of...