VALIDATION OF HPLC AND UV VISIBLE METHODS FOR FEW SELECTED BLOOD PRESSURE LOWERING DRUGS AND THEIR FORMULATIONS
Journal Title: Universal Journal of Pharmaceutical Research - Year 2017, Vol 2, Issue 1
Abstract
A simple, precise and accurate RP-HPLC method has been developed and subsequently validated for simultaneous estimation of Aliskiren Hemifumarate and Nicardipine Besylate from their combination dosage form. Aliskiren and Nicardipine are widely used antihypertensive drugs at present but their analytical methods are very costly and very complex to simplify the methods with increasing sensitivity new methods were developed which are simple, precise, eco-friendly, less time consuming, rapid and fast and economically chief. First standard curve was plotted then the method is validated by using recovery studies, linearity, correctness and reproducibility, robustness, ruggedness, detection limit, quantification limits, stability studies etc. The validated technique has been with success used for stress testing analysis of Aliskiren and Nicardipine. The stress testing studies revealed that the tactic was with success utilized to resolve the degraded product from the sample. From the peak purity profile it had been demonstrated that there was no interference of degradation product and the purity of angle were found to be but the purity of threshold. This work was undertaken with an aim of developing HPLC and Specrophotometric techniques for analysis of Aliskiren and Nicardipine. Number of trials was taken for selection of column and M. Phase’. The proposed method was validated as per the ICH and USP guidelines.
Authors and Affiliations
Shahul Hameed M, Jat R K, Indulatha V N
Keywords
Related Articles
- EP ID EP36379
- DOI https://doi.org/10.22270/ujpr.v2i1.R6
- Views 327
- Downloads 0
WOUND HEALING ACTIVITY OF JATROPHA TANJORENSIS LEAVES
The term “wound” is defined as a disruption of normal anatomical structure. Therefore, “healing” is the complex and dynamic process that results in the restoration of anatomical continuity and function. Albino Wistar rat...
INTERLEUKIN-22 SERUM LEVELS IN PATIENTS WITH RHEUMATOID ARTHRITIS IN SANA'A CITY, YEMEN
Interleukin (IL) -22 is a novel mediator of a member of IL-10 family cytokines that is produced by many different types of lymphocytes including both those of innate and adaptive immune system. This cytokine has potent p...
FORMULATION AND IN-VITRO EVALUATION OF FLOATING MICROBALLOONS OF STAVUDINE
Objective of present study involves preparation and evaluation of floating microballoons of Stavudine. It is a potent antiviral agent, used for treatment of human immunodeficiency virus (HIV) infection. In present study...
INVESTIGATING THE RELATION OF TALENT MANAGEMENT WITH DEVELOPING SUCCESSORS OF HUMAN POWER OF KERMAN UNIVERSITY OF MEDICAL SCIENCES
Today, financial and technological resources are not only enumerated the organization advantage but possessing capable and mighty people may also compensate for lack or imperfection of other resources. In the present co...
COMPARATIVE ANALYSIS OF THE PHYTOCHEMICAL COMPOSITIONS OF LEAF, STEM-BARK AND ROOT OF AZADIRACHTA INDICA (NEEM)
Plant chemicals abound in different parts of plants, in different compositions. Thus, the comparative screening of the leaf, stem-bark and root of Azadirachta Indica becomes imperative. The presence of nine phytochemical...