Vancomycin Resistance among Methicillin Resistant Staphylococcus Aureus Isolates From Tertiary Care Hospital
Journal Title: International Journal of Health Sciences and Research - Year 2017, Vol 7, Issue 1
Abstract
Background: Staphylococcus aureus is one of the most common causes of nosocomial infections. Vancomycin is the drug of choice to treat infections caused by MRSA. Objectives: The study determines Vancomycin MIC of MRSA strains by both agar dilution and Vitek methods. Presence of VAN a gene was also determined. Materials and Methods: Study was conducted from April 2015-March 2016.A total 70 Staphylococcus aureus strains were included. Methicillin resistance was determined by using Cefoxitin disc (30ug) as per CLSI guidelines. Vancomycin resistance was detected by both agar dilution and Vitek methods. PCR was done to determine presence of VAN A gene among VISA strains. Results: Among 70 S.aureus strains, 40 (57%) strains were methicillin resistant. Maximum MRSA strains (55%) were isolated from pus samples. 6 VISA (Vancomycin intermediate S. aureus strains) strains were detected by agar dilution method. Vitek was able to detect 3 VISA strains. All VISA strains were sensitive to Rifampicin and Linezolid by disc diffusion method. All VISA strains were negative for VAN A gene by PCR. Conclusion: Disc diffusion method misclassifies vancomycin intermediate isolates as fully susceptible strains. Clinical laboratories must perform MIC method to correctly identify VISA strains and avoid treatment failure.
Authors and Affiliations
Thakar V
Keywords
Related Articles
- EP ID EP368075
- DOI -
- Views 125
- Downloads 0
Effectiveness of School-Based, Peer-Led Sexual Health Interventions in Increasing STIS/HIV Knowledge amongst Adolescents: A Systematic Review
Background: The spread of sexually transmitted infections (STIs) including HIV and early pregnancy among adolescents have become public health problems in many settings. School-based, peer-led sexual health education aim...
A Hospital-Based Clinical Study of Cutaneous Changes in Overweight and Obesity
Background: Obesity has been considered as one of the major epidemics of 21st century due to its detrimental effects on homeostatic milieu of the body. Not only internal organ systems but externally our so called externa...
Perceived Satisfaction towards Utilization of Maternal and Child Health Care Services Among Beneficiaries Attending District Public Health Care Facility of Haryana
The health of mother and child constitutes one of the major serious concerns. Health care service utilization is a concept of expressing the extent of interaction between the service and the beneficiaries for whom it is...
Pictorial Health Warnings on Tobacco Packs- A Knowledge, Attitude and Practice Survey among Indian Engineering Students
Objectives: To assess the knowledge, attitude and practice about the Pictorial health warnings on tobacco packs among engineering students and identify the areas that need consideration Methods: Four Hundred engineering...
Modified Alvarado Scoring System as a Diagnostic Tool in a Central Indian Rural Hospital
Acute appendicitis is the most common cause of acute abdomen which compels the patient to seek medical attention and appendectomy is most commonly performed operation in acute abdomen. Lifetime risk of acute appendicitis...