Vancomycin Resistance among Methicillin Resistant Staphylococcus Aureus Isolates From Tertiary Care Hospital
Journal Title: International Journal of Health Sciences and Research - Year 2017, Vol 7, Issue 1
Abstract
Background: Staphylococcus aureus is one of the most common causes of nosocomial infections. Vancomycin is the drug of choice to treat infections caused by MRSA. Objectives: The study determines Vancomycin MIC of MRSA strains by both agar dilution and Vitek methods. Presence of VAN a gene was also determined. Materials and Methods: Study was conducted from April 2015-March 2016.A total 70 Staphylococcus aureus strains were included. Methicillin resistance was determined by using Cefoxitin disc (30ug) as per CLSI guidelines. Vancomycin resistance was detected by both agar dilution and Vitek methods. PCR was done to determine presence of VAN A gene among VISA strains. Results: Among 70 S.aureus strains, 40 (57%) strains were methicillin resistant. Maximum MRSA strains (55%) were isolated from pus samples. 6 VISA (Vancomycin intermediate S. aureus strains) strains were detected by agar dilution method. Vitek was able to detect 3 VISA strains. All VISA strains were sensitive to Rifampicin and Linezolid by disc diffusion method. All VISA strains were negative for VAN A gene by PCR. Conclusion: Disc diffusion method misclassifies vancomycin intermediate isolates as fully susceptible strains. Clinical laboratories must perform MIC method to correctly identify VISA strains and avoid treatment failure.
Authors and Affiliations
Thakar V
Keywords
Related Articles
- EP ID EP368075
- DOI -
- Views 127
- Downloads 0
Evaluation of Bioethics Workshop Using Pre- and Post-Test Questionnaire
Background: The previous studies reported a lack of awareness about bioethics among the medical undergraduate students and teaching staff. Hence, this study was planned to promote Bioethics through a workshop and aimed t...
Evidence on the Association between Socioeconomic Resources and Amenable Mortality
Background: There is growing interest in finding an indicator for health system performance that is directly related to health system expenditure and improvements in population health. This paper analyses the relationshi...
Pregnancy Outcome in Women with Hypothyroidism
Objective: The present study was to know the pregnancy outcome in women with hypothyroidism. Methods: This randomized prospective study was carried out in the department of Obstetrics and Gynecology, Kamla Nehru State H...
Health Related Quality of Life (HRQoL) Among Patients with Lymphatic Filariasis
Health Related Quality of Life (HRQoL) measures assesses a person’s perception about their physical, mental and social well being in their daily life. Lymphatic Filariasis is a major public health problem and the two maj...
Hazardous Effect of Viruddha Ahara (Incompatible Food) on Health: A Critical Review
The wellbeing of the individual chiefly depends upon the kind of food he consumes. The maximum benefits of Ahara (food) can be achieved only by following the healthy dietary guidelines mentioned in classics of Ayurveda w...