Vancomycin Resistance in Clinical Isolates of Methicillin Resistant Staphylococcus aureus from a Tertiary Care Hospital
Journal Title: Saudi Journal of Pathology and Microbiology - Year 2018, Vol 3, Issue 10
Abstract
Staphylococcus aureus is one of the most common causes of Blood Stream infections (BSI), skin and wound infections, osteomyelitis, endocarditis, and nosocomial infections, especially pneumonia, surgical site infections (SSI), and continue to be a major cause of community-acquired infections. Methicillin Resistant Staphylococcus aureus (MRSA) is an important cause of community and hospital acquired infections. MRSA are mainly nosocomial and are increasingly reported from many countries worldwide. The purpose of present study was to determine the sensitivity of S. aureus isolated from infected patients to methicillin and to evaluate the possible presence of VRSA in our tertiary care hospital. Staphylococci were isolated and identified by standard microbiological procedures. Methicillin resistance was detected by using cefoxitin (30 µgm) by disc diffusion method. MRSA strains detected were then subjected to vancomycin agar screen test and E test to detect vancomycin resistance. Out of the total 500 S.aureus isolates, methicillin resistance was observed in 47.4% of isolates. . By E-test, 24.1 % MRSA isolates had Vancomycin MIC value of 0.75 and 21.9% of MRSA had Vancomycin MIC value of 0.5. Only one isolate had MIC value of 2.
Authors and Affiliations
Aparna Yadav, Dr Akansha Sharma, Antariksh Deep
Keywords
Related Articles
- EP ID EP484281
- DOI -
- Views 78
- Downloads 0
Yoghurt Production from Powdered Milk using Mixed Lactic Acid Bacteria Starter Cultures
Abstract: Lactobacillus bulgaricus, Streptococcus thermophilus and Lactobacillus fermentumisolated from fermented foods: yoghurt, “ugba” and “kunu-zaki” using MRS agar and a commercially acquired lactic acid bacteria (LA...
Breast Stromal Periductal Sarcoma: A Case Report
Periductal stromal sarcoma is a rare lesion. It was considered to be a variant of phyllode tumor whereas they are morphologically different. Our patient was a 39 years old, with no previous history, who presented a nodul...
Prevalence and risk factors of panton valentine leukocidin-producing Staphylococcus aureus in Iran
Abstract: Staphylococcus aureus (S. aureus) causes a wide spectrum of clinical disorders with pathogenic factors such as various toxins. The aims of this study were analysis of the prevalence of pvl gene among MSSA, MRSA...
Antifungal Activity of Moringa stenopetala (Baker f.) Cufod Against Alternaria helianthi
Abstract: Sunflower is an important oil seed crop in India. The crop is susceptible to various diseases among which leaf blight caused by Alternaria helianthi is more destructive. The present study aimed at determining a...
Serum Transferrin Receptor Assay in the Diagnosis of Iron Deficiency Anemia in Children
The present aimed to assess the utility of serum transferrin receptor (sTfR) and sTfR -ferritin index in diagnosing iron deficiency anemia (IDA) in children.Ninety children≤ 12years of age with anemia (6-59 months; Hb<11...