Variability of Phenolic Compounds and Biological Activities among Wormwood Extracts Originated from Different Bioclimatic Zones
Journal Title: Journal of Advances in Biology & Biotechnology - Year 2017, Vol 15, Issue 2
Abstract
Aim: Wormwood is an aromatic spice widely used as flavor, antioxidant and antimicrobial agents. In this study the variability of total phenolics, total flavonoids, antioxidant, antibacterial and antifungal activities among leaves extracts originated from four Tunisian regions were assessed. Study Design: All the analyses were achieved in triplicate. Methodology: Fresh leaves of Artemisia absinthium L. were harvested from four regions of Tunisia representing four different bioclimatic areas ranging from humid to arid zone. Phenolic and flavonoid contents were assessed based on colorimetric methods. The antioxidant activities of the studied extracts were tested based on DPPH and ABTS methods. The antibacterial and antifungal activities of Artemisia absinthium phenolic extracts were determined against ten indicators microorganisms by measuring the diameter of the growth-inhibition zone in millimeters, the minimum inhibitory concentration and the minimum bactericidal concentration. Results: Considerable levels of bioactive phenolic compounds were revealed for the studied extracts. The highest levels of phenolic compounds were recorded for Gafsa site (Total Phenolic Content=127.5±5.22 mg GAE/g DW, Total Flavonoid Content=34.26±2.48 mg RE/g DW) while the lowest contents were observed for Ghar Dimaou locality (Total Phenolic Content =94.23±4.81 mg GAE/g DW, Total Flavonoid Content=26.95±1.98 mg RE/g DW). Similarly, considerable levels of antioxidant capacities were revealed based on both DPPH and ABTS tests with a significant variation according to the studied site. All the investigated extracts presented antimicrobial capacities against the ten tested strains with a variation according both to the extract origin and to the tested microbial strain. Especially, the highest toxicity was recorded against the species Aspergillus niger (MIC: 3.12 µg/ml, MBC: 6.25 µg/ml) and Candida albicans (MIC: 6.25 µg/ml, MBC: 12.5 µg/ml). Comparable levels were observed for bacterial strains with slight more effectiveness against Gram positive strains.
Authors and Affiliations
Hanene Ghazghazi, Chedia Aouadhi, Imen Klay, Hanen Chakroun, Leila Riahi
Keywords
Related Articles
- EP ID EP318096
- DOI 10.9734/JABB/2017/36144
- Views 106
- Downloads 0
Lecithin Isolated from Melon Seed Oil: Antioxidant Activity
Context: A number of antioxidants have been proposed for certain products; however, little information is available about the efficacy of vegetable oil lecithin as antioxidant in food products. Aim: The study was design...
In vivo Evidence of Mice Hippocampal Alterations Resulting from High Dose Caffeine Exposure during Intrauterine Life
Caffeine ingestion during pregnancy requires attention and investigation towards assessing the level of safety and possible effects on the brain and mental health. Caffeine ingestion during pregnancy has been discouraged...
Biotechnology as a Cradle of Scientific Development: A Review on Historical Perspective
Historically, the science of biotechnology started through domestication of plants and animals by early men and selective plant breeding which subsequently led to transgenesis. Fermentation of grains and fruits using yea...
Statistical Optimization of Fermentation Parameters for Cellulase Production Utilizing Banana Peel
Aim: To optimize the physical parameters of solid state fermentation (SSF) by implementing statistical tool for production of cellulase, one of the potential biocatalyst in industries. Study Design: Fermentation was carr...
Isolation and Characterization of Bioflocculant-Producing Bacteria from Wastewater at Jimeta, Adamawa State
Aim: The aim of this study is to isolate, identify and screen for bacteria capable of producing bioflocculant from three wastewater disposal sites; Jimeta Abattoir, Jimeta Modern Market and the Gwari Market waste disposa...