Variant Antigens Characterize Pathologic Immune Complexes in Severe Plasmodium falciparum Malaria

Journal Title: Journal of Medical Science And clinical Research - Year 2014, Vol 2, Issue 12

Abstract

Introduction: Plasmodium falciparum infection is characterized by deadly complications such as severe malaria-associated anaemia (SMA) and cerebral malaria (CM). The exact mechanisms underlying pathogenesis of these severe forms of Plasmodium falciparum malaria are not fully understood yet they are associated with a lot of morbidity and mortality. Studies have shown a link between severe P. falciparum malaria and levels of circulating immune complexes (CIC) but the exact role of these CICs and the specific malarial antigens involved in the pathogenesis of severe P. falciparum malaria is still unclear. Objectives: This study aimed to investigate the qualitative differences in P. falciparum antigens in serum immune complexes (ICs) between children with the severe forms of Plasmodium falciparum malaria and those with uncomplicated malaria. It was aimed at identifying and characterizing the predominant P. falciparum antigens that contribute to IC formation in these clinical groups. Methods: ICs were purified using polyethylene glycol (PEG) precipitation and dissociated using an acidic buffer (Glycine-HCL pH 2.0). These were then electrophoresed on one-dimensional and two-dimensional polyacrylamide gel blotted by Western transfer and revealed using human hyperimmune sera. Results: Six distinct P. falciparum antigens were found to be associated with severe malarial anaemia while another three antigens were associated with cerebral malaria when compared to their respective controls. An antigen with approximately 91 kDa was highly associated with SA (P < 0.01) while a slightly lighter antigen of about 87 kDa was significantly associated with CM (P < 0.01). Conclusion: These findings may point to differences in qualitative characteristics of ICs in children with SMA and CM and give insight into potential mechanisms of the disease. The findings further suggest differing target of humoral immunity in the severe forms of malaria.

Authors and Affiliations

Erick K Mibei

Keywords

Related Articles

Systemic Lupus Erythematosis and Adverse Pregnancy out Come - A Prospective Study

Background: Systemic lupus erythematosus (SLE) is one of the most common autoimmune disorders that affect women during their childbearing years. It is an idiopathic chronic inflammatory disease that affects skin, joints,...

Study of Pap Smear with Revised Bethesda System of Reporting, in Screening of Ca Cervix, in Patients Attending in Tertiary Care Hospital at Darbhanga, Bihar

Objective: Carcinoma cervix is the most common carcinoma of female. India is said to be the capital of cancer cervix because of highest incidence rate and mortality rate. It is more prevalent in rural area. In urban area...

Clinical Profile of Patients with Diabetic Foot Ulcer and Its Correlation with Neuropathy, Peripheral Vascular Disease and Glycemic Control

Diabetes is one of the most prevalent chronic diseases. It is estimated that the annual Population based incidence of a diabetic foot ulcer ranges from 1.0 % to 4.1 %. The lifetime Incidence may be as high as 25%. 85% of...

Endo- Perio Lesion- A Brief Review

Endo- Perio lesions are considered to be complex dental conditions for the clinicians to establish accurate diagnosis and treatment plan. The aim of this article is to bring about proper diagnosis followed by the removal...

Evaluation of Dental Caries Awareness among the Parents of School Going Children

AIM: The purpose of this study is to evaluate the awareness of parents about the dental caries among the school going children in chennai. OBJECTIVES: The objective of this study is to investigate the dental caries aware...

Download PDF file
  • EP ID EP208033
  • DOI -
  • Views 69
  • Downloads 0

How To Cite

Erick K Mibei (2014). Variant Antigens Characterize Pathologic Immune Complexes in Severe Plasmodium falciparum Malaria. Journal of Medical Science And clinical Research, 2(12), 3103-3118. https://europub.co.uk/articles/-A-208033