Vascular Endothelial Growth Factor and Thrombospondin-1 mRNA Expression in Bladder Tumors: Correlation with Histopathology and Prognosis
Journal Title: Üroonkoloji Bülteni - Year 2019, Vol 18, Issue 1
Abstract
Objective: The purpose of this study was to determine genetic expression levels of vascular endothelial growth factor (VEGF) and thrombospondin-1 (TSP-1) in fresh bladder tumor specimens and evaluate their relationship with tumor histopathological features and their prognostic value in recurrence and progression in patients with bladder cancer. Materials and Methods: Patients who were treated for urothelial cancer of the bladder and followed for at least 6 months were included in this retrospective study. Following RNA isolation from fresh tumor tissue samples recovered from transurethral resection or radical cystectomy specimens, VEGF and TSP-1 mRNA expression was analysed by reverse transcription polymerase chain reaction (RT-PCR). The findings were examined in relation to the histopathological parameters and recurrence and progression rates of the respective tumors. Results: Sixty-eight patients were included in the study. Mean follow-up time was 22.6 months. In patients with non-muscle-invasive urothelial bladder cancer (NMIBC), rates of recurrence and progression were 64% and 35%, respectively. RT-PCR analyses revealed VEGF mRNA expression in 29 patients (43%) and TSP-1 mRNA expression in 22 patients (32%). Recurrence and progression were observed during follow-up in 64% and 24% of the 25 NIMBC patients with positive VEGF expression, while these rates were 63% and 30% among the 30 NIMBC patients with no VEGF expression, respectively. Rates of recurrence and progression during follow-up were 70% and 30% among NIMBC patients with positive TSP-1 expression and 60% and 26% among patients with no TSP-1 mRNA expression, respectively. Conclusion: In this study, VEGF and TSP-1 mRNA expression was not associated with histological grade or stage of bladder cancer. There was no difference in VEGF expression in tumor tissues from NIMBC patients with or without disease recurrence. Though lacking statistical significance, a positive correlation between TSP-1 expression and tumor recurrence and progression was seen among the NIMBC patients in our study. Although stimulatory and inhibitory factors are known to regulate angiogenesis, no definitive conclusions have been reached regarding their mechanism of action or the prognostic significance of their up-or down-regulation.
Authors and Affiliations
Bora Özveren, Levent Türkeri
Keywords
Related Articles
- EP ID EP550865
- DOI 10.4274/uob.galenos.2018.1179
- Views 95
- Downloads 0
The Efficiency of Docetaxel Chemotherapy on Castration Resistant Prostate Cancer: Singe Center Experience
Objective: Prostate cancer (PCa) is the second most commonly seen cancer type worldwide. Mortality due to PCa is mostly linked to the development of castration resistant PCa (CRPC). In these patients, the main treatment...
Comparison of Lidocaine and Prilocaine Efficiencies in Periprostatic Nerve Block for Transrectal Prostate Biopsy: A Randomized Prospective Study
Objective: Periprostatic nerve block (PPNB) and intrarectal local anesthesia (IRLA) are two of the most commonly used methods for pain control in the transrectal ultrasonography (TRUS)-guided prostate needle biopsy proce...
Contemporary Trends in Adjuvant and Neoadjuvant Treatment for Renal Cell Carcinoma
Renal cell carcinoma is an increasingly significant cancer in which surgical resection is still the sole curative approach. There is a risk of recurrence in one-third of patients after surgery. Successful experiences wit...
Impact of Delay from Biopsy to Surgery on the Rate of Adverse Pathologic and Oncologic Outcomes for Clinically Localized Prostate Cancer
Objective: Due to the widespread usage of prostate-specific antigen screening, the number of patients diagnosed with prostate cancer is steadily increasing. Many factors such as high operating room demand, insurance reim...
Synchronous and Metachronous Secondary Tumors of Bladder Cancer Patients
The improvements in cancer treatment prolonged survival in patients. Despite this survival benefit, chemotherapies, radiotherapies or combination therapies, and continuing exposure to the same carcinogenic agents may lea...