Viability and phenotypic heterogeneity of Rhodococcus Rhodochrous CNMN-AC-05 in the presence of fullerene C60
Journal Title: One Health & Risk Management - Year 2021, Vol 2, Issue 3
Abstract
Introduction. In recent years, due to wide applications of nanotechnologies in various fields, the safety of nanomaterials has become a pressing issue. Fullerene C60 is not an exception. Research on the activity of microorganisms and their interaction with nanoparticles is of major importance, both for microorganisms and for the ecosystem as a whole. Material and methods. Fullerene C60powder was purchased from Sigma-Aldrich. The object of study was R. rhodochrous CNMN-Ac-05 strain. The number of viable bacterial cells was estimated by colony-forming units (CFU). The morphological features of the rhodococci colonies have been described according to the usual microbiological method. Results. It was established that fullerene C60 in concentrations of 1-25 mg/L fullerene C60 stimulated the growth of R. rhodochrous by 2.4-2.8 times. As the concentration of fullerene C60 increased up to 50-100 mg/L, the multiplication and growth of rhodococci decreased by 29.5% and 38% respectively. In the presence of 1-10 mg/L fullerene C60 the rhodococci population remained homogeneous, being composed of 100% S type colonies. The increase of fullerene C60 concentration led both to the decrease in the CFU number and to the appearance of R type colonies, up to 1.3% of population. Conclusions. Fullerene C60 in concentrations 1-100 mg/L had no obvious toxic effect on the rhodococci strain. The optimum concentration is 10 mg/L. The concentrations higher than 25 mg/L led to the dissociation of rhodococcal population and diminution in the CFU counts, but not to the total inhibition.
Authors and Affiliations
Olga POSTOLACHI, Inna RASTIMESINA, Valentina JOSAN
Keywords
Related Articles
- EP ID EP696228
- DOI 10.38045/ohrm.2021.3.01
- Views 102
- Downloads 0
The lichen genus Lepraria Ach. (Stereocaulaceae, lichenized Ascomycota) in Romania
Introduction. The genus Lepraria includes diffuse or indefinitely delimited species, always sterile, Lendemer (1) naming it „the most unusual member of the sterile lichen crusts”. According to Tonsberg (2), the leprarioi...
Retrospective study on the prevalence of COVID-19 confirmed cases and evidence of gender bias in Libya
Introduction. To stop SARS-CoV-2 from spreading, it's crucial to have a solid understanding of the baseline characteristics of demographic variables, clinical symptoms, and comorbidity so that quarantining and testing pr...
The efficacy of direct-acting antiviral therapy in patients with chronic hepatitis C
Introduction. Viral hepatitis C (HCV) is a significant global health problem. The risk of developing chronic HCV is up to 80% of patients, of whom 10-20% can develop liver cirrhosis or hepatocellular carcinoma which can...
Analysis of clinical and molecular genetic characteristics of Wiskott-Aldrich syndrome and X-linked thrombocytopenia
Introduction. Wiskott-Aldrich syndrome is a rare X-linked disorder characterized by microthrombocytopenia, eczema, and recurrent infections. It is caused by mutations of the WAS gene which encodes the WAS protein (WASp)...
Epidemiological situation regarding the new type of coronavirus infection (COVID-19) among healthcare workers in the Republic of Moldova
Introduction. The emergence of the new coronavirus (SARS-CoV-2) has led to a rapid spread of novel infection (COVID-19) worldwide. Since February 1, 2021, to date, more than 103,989,900 cases and more than 2 million deat...