Viability of Escherichia coli ATCC 8739 in Nutrient Broth, Luria-Bertani Broth and Brain Heart Infusion over 11 Weeks
Journal Title: Electronic Physician - Year 2013, Vol 5, Issue 1
Abstract
Abstract: Background: Escherichia coli is a widely studied prokaryotic system. A recent study had demonstrated that reduced growth of E. coli after extended culture in Luria-Bertani broth is a result of depletion of fermentable sugars but able to sustain extended cell culture due to the presence of amino acids, which can be utilized as a carbon source. However, this had not been demonstrated in other media. The study aimed to determine the growth and viability of E. coli ATCC 8739 in 3 different media, Nutrient Broth (NB), Brain Heart Infusion (BHI) and Luria-Bertani Broth (LB) over 11 weeks. Methods: Growth of E. coli ATCC 8739 was determined by optical density. Viability was determined by serial dilution/spread-plate enumeration. After 11 weeks, the media were exhausted by repeated culture. Glucose was added to the exhausted media to determine whether glucose is the growth-limiting factor. Results: Our results showed that cell density in all 3 media increased to about 1 x 109 cells/ml by the end of week 1, from the inoculation density of 2.67 x 105 cells/ml, peaked at about 1 x 1013 cells/ml at week 4, before declining to about 5 x 107 cells/ml at week 7. Cell density is highly correlated to genomic DNA content (r2 = 0.93) but poorly correlated to optical density (r2< 0.2). Our results also showed that the spent media were able to support further growth after glucose-supplementation. Conclusion: NB, LB and BHI are able to support extended periods of culture and glucose depletion is the likely reason for declining cell growth.
Authors and Affiliations
Samuel Low, Zhen Aw, Bryan Lin Loo, Kun Lee, Jack Oon, Chin Lee, Maurice Ling
Keywords
Related Articles
- EP ID EP146323
- DOI -
- Views 103
- Downloads 0
The Impact of Hemodialysis on Sexual Function in Male Patients using the International Index of Erectile Function Questionnaire (IIEF)
INTRODUCTION: Routine hemodialysis is one of the preferred treatment methods in patients with chronic kidney disease. It seems that routine hemodialysis can be effective in improving sexual function in these patients. Th...
Quality of bedside teaching in internal wards of Qaem and Imam Reza hospitals in Mashhad.
Bedside teaching is a patient-based teaching method in medical education. The present study has been conducted with the aim of investigating the quality of bedside teaching in the internal wards of Qaem and Imam Reza Edu...
Nanocomposites in food packaging applications and their risk assessment for health
Nanotechnology has shown many advantages in different fields. As the uses of nanotechnology have progressed, it has been found to be a promising technology for the food packaging industry in the global market. It has pro...
Breast cancer chemopreventive and chemotherapeutic effects of Camellia Sinensis (green tea): an updated review
INTRODUCTION: Camellia sinensis belongs to the plant family of Theaceae, native to East Asia, the Indian Subcontinent and Southeast Asia, but naturalized in many parts of the world. The aim of this study was to overview...
The Clinicopathologic Characteristics and 5-year Survival Rate of Epithelial Ovarian Cancer in Yazd, Iran.
Ovarian cancer is the second most common malignancy in women, the most common cause of gynecologic cancer deaths, and most patients have advanced stage disease at the time of diagnosis. The purpose of this study was to e...