Viral metagenomics analysis of poultry faeces in live poultry market, Haikou, China
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2018, Vol 11, Issue 10
Abstract
Objective: To conduct in-depth study of the distribution and diversity of viruses in poultry is of great importance in monitoring the emergence of interspecies transmission of novel viruses that may cause epidemics with public health significance. Poultry is an economically important source of meat, eggs and feathers which plays an important role as natural reservoirs of many pathogenic viruses. Compared with wild animals, poultry have more frequent interactions and therefore opportunities to transmit their viruses to human. Methods: To study the viromes of different types of poultry in Hainan, China, we used metagenomics for deep viral nucleic acid sequencing of the faecal samples collected from chickens, ducks and pigeons from a live poultry market in Haikou. Result: The poultry viromes were identified by sequence similarity comparisons of viral reads (BLASTxE score, <5) against viral reference database. A total of 15 309 viral reads were obtained, approximately 13 063, 1 370 and 876 viral reads were generated from the chicken, duck, pigeon faecal samples, respectively. The majority of the sequences were homologous to the animal virus of Adenoviridae, Herpeaviridae, Picobirnaviridae, Reoviridae, Retroviridae, Circoviridae, Paramyxoviridae, Astroviridae, Caliciviridae, Coronaviridae, Picornaviridae, and Orthomyxoviridae. The VP4 and VP7 segments of a pigeon rotavirus, similar to fox rotavirus in group A, were sequenced and phylogenetically analyzed. The near full genome of a pigeon circovirus was also analyzed. Conclusion: The major types of poultry in a Haikou harbor many different families of viruses in their feces which may have the potential for interspecies transmissions. Further studies should be conducted to identify the most prevalent and important viruses among a larger number of poultry in Haikou and other areas in Hainan.
Authors and Affiliations
Fei-fei Yin
Keywords
Related Articles
- EP ID EP402101
- DOI 10.4103/1995-7645.243109
- Views 73
- Downloads 0
Promising antileishmanial effectiveness of doxorubicin and Doxil against Leishmania major: An in vitro assay
Objective: To evaluate the effect of doxorubicin and its pegylated liposomal formulation (Doxil, Caelyx) on in vitro susceptibility of promastigote and amastigote stages of Leishmania major. Methods: Throughout in vitro...
Zika virus is arriving at the American continent
Zika virus (ZIKV) is the last arbovirosis to arrive to the American continent, following dengue virus (DENV) in 1990, West Nile virus (WNV) in 1999 and chikungunya (CHIK) at 2013. At this time, it represents a pandemic a...
Chemical composition of Mentha suaveolens and Pinus halepensis essential oils and their antibacterial and antioxidant activities
Objective: To determin the chemical compounds of Mentha suaveolens (M. suaveolens) and Pinus halepensis (P. halepensis) essential oils (Eos) and evaluate their antioxidant and antibacterial activities. Methods: The chemi...
An overview on cardioprotective and anti-diabetic effects of thymoquinone
Thymoquinone (TQ), one of the active components of Nigella sativa exhibited to have many biological effects. Several beneficial effects of TQ such as its antidiabetic, antioxidant, anticancer, hypolipidemic, and anti-inf...
Expression of vimentin and glial fibrillary acidic protein in central nervous system development of rats
Objective: To investigate the distribution and contents of vimentin (Vim) and glial fibrillary acidic protein (GFAP) immunoreactivities in the central nervous system (CNS) of normal newborn, adult and aged rats. Methods:...