Virulence and antibiotic resistance of Escherichia coli isolated from rooks
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2013, Vol 20, Issue 2
Abstract
With regard to antibiotic resistance studies in various model animals in the urban environment, the presented study focused on the rook, many behavioural and ecological aspects of which are important from an epidemiological point of view. A total of 130 [i]Escherichia coli[/i] strains isolated from rook faeces during a two-year period (2011–2012) were investigated for antibiotic resistance and virulence. Resistance to ampicillin (60%) and streptomycin (40%) were the most frequent, followed by resistance to fluoroquinolones (ciprofloxacin-22% and enrofloxacin-24%), tetracycline (18%), cotrimoxazol (17%) and florfenicol (14%). Ceftiofur resistance occured in 10.7% of strains and cefquinom resistance in 1.5% of strains. Twenty-five [i]E.coli[/i] strains with a higher level of MICs of cephalosporins (over 2mg/L of ceftazidime and ceftriaxon) and fluoroquinolones were selected for detection of betalactamase genes (CTX-M, CMY), plasmid-mediated quinolone resistance qnrS, integrase 1, and for APEC (avian pathogenic [i]E.coli)[/i] virulence factors ([i]iutA, cvaC, iss, tsh, ibeA, papC, kpsII[/i]). Genes of CTX-M1, CMY-2, integrase 1, [i]papC[/i], [i]cvaC, iutA[/i] were detected in one strain of [i]E.coli[/i], and qnrS, integrase 1, [i]iss[/i], [i]cvaC[/i], [i]tsh[/i] were detected in another [i]E.coli[/i]. DNA microarray revealed the absence of verotoxin and enterotoxin genes and pathogenicity islands. The results show that rooks can serve as a reservoir of antibiotic-resistant [i]E. coli[/i] with avian pathogenic virulence factors for the human population, and potentially transmit such [i]E.coli[/i] over long distances.
Authors and Affiliations
Vladimir Kmet, Zuzana Drugdova, Marta Kmetova, Michal Stanko
Keywords
Related Articles
- EP ID EP80613
- DOI -
- Views 149
- Downloads 0
Prevalence of diabetes mellitus and IFG in the prospective cohort ‘PONS’ study – baseline assessment
Objective: The aim of this cross-sectional study was to assess the prevalence of diabetes mellitus and impaired fasting glucose (IFG) in the Polish-Norwegian Study (PONS) population in Poland.Methods: The presented resul...
Cognitive functions, lipid profile, and Apolipoprotein E gene polymorphism in postmenopausal women
The objective of the study was investigation of the relationship between cognitive functions and lipid profile, BMI and change of body weight in postmenopausal women carriers of Apolipoprotein E gene polymorphisms (APOE)...
Professional communication competences of paramedics – practical and educational perspectives
[b][/b]Introduction: Dissonance between the high ‘technical’ competences of medical professionals, including paramedics or emergency medical technicians (EMT), and the relatively low level of patient satisfaction with ca...
Analysis of expression and amino acid sequence of the allergen Mag 3 in two species of house dust mites-Dermatophagoides farinae and D. pteronyssinus (Acari: Astigmata: Pyroglyphidae).
In the 90's of the XX century, 2 new and important allergens of house dust mites mites were cloned and sequenced: Mag 1 and Mag 3. However, the second allergen has been identified to date only in extracts of Dermatophago...
Managing water safety in healthcare. Part 2 – Practical measures and considerations taken for waterborne pathogen control
These summaries form the second part of presentations made at a recent conference held at the Royal Society for Public Health in London, 16-17 May 2012, on the latest developments in combating waterborne hospital-acquire...