Virulence and antibiotic resistance of Escherichia coli isolated from rooks
Journal Title: Annals of Agricultural and Environmental Medicine - Year 2013, Vol 20, Issue 2
Abstract
With regard to antibiotic resistance studies in various model animals in the urban environment, the presented study focused on the rook, many behavioural and ecological aspects of which are important from an epidemiological point of view. A total of 130 [i]Escherichia coli[/i] strains isolated from rook faeces during a two-year period (2011–2012) were investigated for antibiotic resistance and virulence. Resistance to ampicillin (60%) and streptomycin (40%) were the most frequent, followed by resistance to fluoroquinolones (ciprofloxacin-22% and enrofloxacin-24%), tetracycline (18%), cotrimoxazol (17%) and florfenicol (14%). Ceftiofur resistance occured in 10.7% of strains and cefquinom resistance in 1.5% of strains. Twenty-five [i]E.coli[/i] strains with a higher level of MICs of cephalosporins (over 2mg/L of ceftazidime and ceftriaxon) and fluoroquinolones were selected for detection of betalactamase genes (CTX-M, CMY), plasmid-mediated quinolone resistance qnrS, integrase 1, and for APEC (avian pathogenic [i]E.coli)[/i] virulence factors ([i]iutA, cvaC, iss, tsh, ibeA, papC, kpsII[/i]). Genes of CTX-M1, CMY-2, integrase 1, [i]papC[/i], [i]cvaC, iutA[/i] were detected in one strain of [i]E.coli[/i], and qnrS, integrase 1, [i]iss[/i], [i]cvaC[/i], [i]tsh[/i] were detected in another [i]E.coli[/i]. DNA microarray revealed the absence of verotoxin and enterotoxin genes and pathogenicity islands. The results show that rooks can serve as a reservoir of antibiotic-resistant [i]E. coli[/i] with avian pathogenic virulence factors for the human population, and potentially transmit such [i]E.coli[/i] over long distances.
Authors and Affiliations
Vladimir Kmet, Zuzana Drugdova, Marta Kmetova, Michal Stanko
Keywords
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