Vitamin E and hCG enhance the immunomodulatory properties of LPS-induced mesenchymal stem/stromal cells
Journal Title: Iranian Journal of Veterinary Science and Technology (IJVST) - Year 2021, Vol 13, Issue 1
Abstract
Mesenchymal Stem/Stromal Cells (MSCs) have been applied to modulate various immune-mediated conditions. Prolonged culture of MSCs in vitro reduces their therapeutic efficacy. Pretreatment of the cells with some chemical agents during in vitro expansion could overcome this limitation. This study intended to determine whether pretreatment of adipose-derived MSCs (ASCs) with Human chorionic gonadotrophin (hCG), a glycoprotein hormone, and Vitamin E, an antioxidant, will improve their immunomodulatory ability. In this regard, ASCs were harvested from human processed lipoaspirate. LPS-induced ASCs were preconditioned with 1 mg of hCG and 600 µM of vitamin E for 24h. TSG-6, COX-2, IL-1β, and IL-6 were assessed at the mRNA level in preconditioned and control groups. ASCs were also co-cultured with peripheral blood mononuclear cells (PBMCs) in vitro to determine the functionality of these cells. Results showed that hCG and vitamin E significantly downregulate the pro-inflammatory COX-2, IL-1β, and IL-6 gene expression, while they did not significantly increase TSG-6 expression. Besides, the co-culturing of pretreated ASCs with PBMCs demonstrated that the amount of PBMCs in treated groups (with hCG and vitamin E) was significantly lower than in control groups. These findings revealed that the preconditioning of ASCs with hCG and vitamin E might enhance their immunoregulatory capacity.
Authors and Affiliations
Sepideh sadat Hosseini,Shadi Mehrzad,Halimeh Hassanzadeh,Mahboubeh Kazemi,Nasser Sanjar moussavi,Madjid Momeni Moghaddam,Hamid Reza Bidkhori,Moein Farshchian,
Keywords
Related Articles
- EP ID EP722384
- DOI -
- Views 54
- Downloads 2
Effects of oxidized glutathione, cysteine and taurine supplementations on motility charateristics of different goat spermatozoa types
This study aimed to determine the influences of antioxidants additives on motility characteristics of different goat spermatozoa types including frozen/thawed, fresh, and chilled samples. Ejaculates from five Shami bucks...
Isolation and genotyping of Clostridium perfringens isolated from healthy and diarrheic dogs
Clostridium perfringens has been known as a cause of diarrhoea in dogs. The aim of this research was isolation C. perfringens by culturing and toxinotyping by PCR molecular method. In this research 151 dogsâ faecal sam...
A survey on the gastrointestinal parasites of exotic companion species in Tehran, Iran
Exotic pet owners, ranging from small mammals to reptiles, comprise a considerable portion of veterinary clients. Parasitic infections are a threat both for the animal health and the health of the owner. This study aimed...
Surgical treatment of right lateral abdominal hernia in a heifer
A lateral abdominal hernia is relatively a less common incidence resulting from abdominal wall defects in cows and heifers than other types of hernias. This is usually an acquired defect, and heifers at the farm and fiel...
Study of Toxic Effects of Oxothiazole Derivative as a New Antibacterial Agent
The spread of antibiotic-resistant bacteria in many humans and animals has driven researches to identify and design novel antibacterial agents. In vitro inhibitory activity of (2E)-2-(4,5-dihydro-4-oxothiazol-2-yl)-2-(th...