Waste Oil Biodegradation Potential among Bacteria Isolates from Waste Oil and Pristine Soils
Journal Title: Biotechnology Journal International - Year 2016, Vol 11, Issue 4
Abstract
The research study was aimed at investigating waste oil biodegradation potential among bacteria isolates from waste oil and pristine soil samples. Auto-mechanic workshop and pristine soil environments were randomly sampled within Calabar Metropolis. The study was completed within a period of six months. Standard microbiological methods were used to isolate, characterize and identify bacteria from the collected soil samples, while waste oil biodegradation potential of the bacteria isolates were determined using screen test for used engine oil and hydraulic oil utilization by bacteria isolates from both waste oil polluted and pristine soil. The level of turbidity, degree of precipitation and colour of precipitate were used to assess the biodegradation ability of the bacteria isolates. The total heterotrophic bacteria counts from the waste oil polluted soil samples ranged from 3.16x106 to 4.15x106cfu/g while that of the pristine soil sample ranged from 1.91x105 to 9.12x105cfu/g. Used engine oil biodegraders from the waste oil polluted soil were identified as Serratia spp, Enterobacter spp while efficient used engine oil and hydraulic oil biodegraders were identified as Shigella spp, Corynecbacterium spp, Klebsiella spp, Yersinia spp, Serratia spp and Aeromonas spp, Bacillus spp and Pseudomonas spp, while Serratia spp and Yersinia spp from the pristine soil also showed used engine oil biodegradation potentials and Bacillus spp was identified as efficient used engine and hydraulic oil biodegraders. However, it was obvious from the study that waste oil degraders and their proportion within an area appear to be marginally higher in environment with waste oil exposure and pollution as confirmed by the biodegradation potentials of bacteria isolates from the auto-mechanic workshop investigated, this therefore calls for the optimization of the process as it could serve as a more eco-friendly approach for the bioremediation of waste oil contaminated sites.
Authors and Affiliations
D. R. Tiku, B. E. Asikong
Keywords
Related Articles
- EP ID EP237759
- DOI 10.9734/BBJ/2016/23598
- Views 117
- Downloads 0
In Vivo Safety Assessment of the Plant Growth-Promoting Rhizobacterium Bacillus cereus RS87 and Rhizo-product
Aims: To assess the acute toxicity and skin irritation potential of a rhizobacterium Bacillus cereus RS87 and the rhizo-product in rats and rabbits. Study Design: Adult Wistar rats were gavaged with a single dose of B....
The Effect of Locally Fabricated Pelletizing Machine on the Chemical and Microbial Composition of Organic Fertilizer
In order to make organic fertilizer more quantifiable and attractive to farmers, a pelletizing machine was fabricated locally to produce pellets from powdery fertilizer. However, concerns have been raised on the potentia...
Biogas Production from Different Biomass Wastes with/without Leachate Recirculation
This study attempts to determine the effect of leachate recirculation on biogas production. The study was carried out by using the co-disposal of three major wastes: municipal solid waste (MSW), sewage sludge and cow dun...
Antibacterial Potential of Magnesium Oxide Nanoparticles Synthesized by Aspergillus niger
A total of 280 urinary tract infection samples were collected in this investigation. Out of them 212(75.7%) samples showed a positive response.to bacterial isolates. Morphological, cultural and biochemical testes were co...
Technological Properties of Lactococcus lactis subsp. lactis I23 Isolated from Nigerian Beef and Characterization of Bacteriocin Produced by It
Aim: The present study reports the phenotypic and molecular characterization of Lactococcus strains isolated from Nigerian beef. The technological properties of selected strains were evaluated in order to test their suit...