Weak association of Staphylococcus aureus hemolytic activity with reduced FEV1 in older children with cystic fibrosis
Journal Title: International Journal of Medical and Health Research - Year 2018, Vol 4, Issue 9
Abstract
This study aimed to determine the role of staphylococcal hemolytic activity in the pathogenesis of cystic fibrosis (CF) lung disease. We collected 50 Staphylococcus aureus isolates and pulmonary function data from CF patients. The hemolytic activities of these isolates and lung functions were analyzed. In addition variation in the hla promoter region of these isolates was determined following PCR amplification and sequencing. Wide ranges of hemolytic activities were observed in these isolates. A weak negative correlation between the hemolytic activities and the FEV1 percent predicted was shown in CF patients greater than ten years old. Moreover, we found a significant difference in the FEV1 percent predicted and FEV1/FVC ratio in S. aureus CF patients older than 10 years of age compared to those less than 10 years of age, suggesting a greater burden of obstructive lung disease in the older group. DNA alignment analysis on the DNA sequences revealed eight single nucleotide polymorphisms (SNPs) within the hla promoter region, which did not correlate with hemolytic activity. Our results suggest that higher levels of hemolytic activity may contribute to the pathogenesis of CF, resulting in lung function deterioration. However, SNPs in different locations of hla promoter regions do not direct hemolytic activity.
Authors and Affiliations
Ying Liu, Lu Ji, Junshu Yang, Dengke Zhong, Jiang Zhang, Mark Wylam, James Phillips, Yinduo Ji
Keywords
Related Articles
- EP ID EP587600
- DOI -
- Views 154
- Downloads 0
To study the association of obstructive airway disease in diabetic and non-diabetic subjects using spirometry
Background: Diabetes mellitus is a chronic illness requiring long term medical attention so as to prevent the development of its devastating complications. Poorly controlled diabetes affects almost every organ of the bod...
Case report: Herpes zoster oticus with cranial polyneuropathies
Herpes zoster oticus is defined as a herpetic vesicular rash on the concha, external auditory canal or pinna with a lower motor neuron palsy of the ipsilateral facial nerve. It accounts for more facial palsies than was p...
Comparison of Ilio-inguinal Ilio-hypogastric nerve block with spinal anesthesia for inguinal hernia repair
Introduction: spinal anaesthesia is most widely employed form of regional anaesthesia for lower abdominal and lower limb surgery. Hernioplasty under local anaesthesia is an acceptable alternative to spinal anaesthesia es...
A study on the association of serum vitamin D and iron level with level of control of childhood asthma
Introduction: Associations between an unhealthy diet and asthma are reported. A recent attention has focused on the possible relationship between vitamin D and iron and the presence of asthma. The aim: To assess the leve...
Analysis of caesarean-section rates at an Urban Municipal Maternity Hospital according to Robson's ten group classification system
Background: Rising caesarean rates have been a matter of concern. The WHO proposes that health care facilities should use the Robson's 10 group classification system to audit their Caesarean sections (CS) rates. This wou...