ZERO, FIRST, SECOND ORDER DERIVATIVE AND AREA UNDER CURVE SPECTROPHOTOMETRIC METHODS FOR DETERMINATION OF CEFIXIME TRIHYDRATE IN PHARMACEUTICAL FORMULATION
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 6
Abstract
Objective: A simple, accurate, precise and specific zero, first, second order and area under curve spectrophotometric methods has been developed for determination of Cefixime Trihydrate in its tablet dosage form by using methanol as a solvent.Methods: (1) Derivative spectrophotometric methods: The amplitudes in the zero order derivative of the resultant spectra at 287 nm, first order derivative of the resultant spectra at 307 nm and second order derivative of the resultant spectra at 307 nm were selected to find out cefixime trihydrate in its tablet dosage form by using methanol as a solvent.(2) Area under curve (Area Calculation): The proposed area under the curve method involves measurement of area at selected wavelength ranges. Two wavelength ranges were selected 281-295 nm for estimation of cefixime trihydrate.Results: The linearity was found to be 5-25 μg/ml for cefixime trihydrate. The mean % recoveries were found to be 100.97%, 100.58%, 99.60% and 101.14% of zero, first, second derivatives and area under curve method of cefixime trihydrate. For Repeatability, Intraday precision, Interday precision, % RSD were found to be 0.2106, 0.2901 and 0.22569,0.2571 for zero order, 0.0008,0.6438 and 5.7700,0.3201 for first order, 5.2358,0.5701 and 0.0003,1.8601 for second order and 4.2571,0.7251 and 0.0582,1.2563 for area under the curve method respectively. Limit of Detection and Quantitation were found to be 0.42μg/ml and 1.35μg/ml for zero order, 0.416μg/ml and 0.952μg/ml for first order, 0.718μg/ml and 2.314μg/ml for second order, 0.819μg/ml and 2.429μg/ml for area under curve method respectively. Assay results of market formulation were found to be 100.97%, 100.58%, 99.60% and 101.14% for zero order, first order, second order and area under the curve method respectively. The proposed method has been validated as per ICH guidelines and successfully applied to the estimation of cefixime trihydrate in its Tablet dosage form.Conclusion: The developed methods can be concluded as accurate, sensitive and precise and can be easily applied to the pharmaceutical formulation.Â
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Audumbar Digambar Mali
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