ZERUMBONE (ZER) INDUCES APOPTOSIS IN HEPG2 CELLS VIA MITOCHONDRIAL PATHWAY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 5
Abstract
Objective: The aim of the study is to determine the anti-cancer effect of ZER on the human hepatocellular carcinoma (HepG2) cell line.Methods: The anti-cancer mechanisms investigated were apoptosis and anti-proliferation. The assays used in the in vitro study were 3-4-5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide (MTT), annexin V and acridine orange/propidium iodide staining, and cell cycle analysis to determine apoptosis. Colorimetric assays were employed for caspase-3 and 9 determinations. The morphological changes were determined by scanning electron microscopy.Results: Zerumbone inhibited the proliferation of HepG2 cells in a dose-dependent manner and induced cell cycle arrest at the G2/M phase and apoptosis, shown by chromatin condensation, cell shrinkage and formation of apoptotic bodies, in the HepG2 cells in a time-dependent manner. Zerumbone also stimulated caspase-3 and-9 activities in the HepG2 cells.Conclusions: This study suggests that the induction of apoptosis of ZER on HepG2 was via the mitochondrial pathway.Â
Authors and Affiliations
Nozlena A Samad, Ahmad Bustamam Abdul, Rasedee Abdullah, Tengku Azmi Tengku Ibrahim, Heshu Rahman, Yeap Swee Keong
Keywords
Related Articles
- EP ID EP578826
- DOI -
- Views 60
- Downloads 0
ANTI QUORUM SENSING POTENTIAL OF MORINGA OLEIFERA SEED EXTRACT
Objective: Most infectious diseases caused by bacteria are known to be regulated by Quorum-sensing mechanisms which in turn regulate the pathogenicity. Evaluation of phytochemicals present in Moringa plant extract for po...
MICROENCAPSULATION OF PULP OF MANGIFERA INDICA L. BY SPRAY DRYING AND ANTIOXIDANT ACTIVITY
Introduction: For the food industry and trade, it is very important to obtain powders based on fruits or vegetables by means of drying techniques that allow them to preserve their nutritional, organoleptic properties and...
EVALUATION OF ANXIOLYTIC ACTIVITY OF METHANOL EXTRACT OF TRACHYSPERMUM AMMI L
Objective: The anxiolytic activity of the methanol extract of Trachyspermum ammi. L was investigated by hole board and passive avoidance response test.Methods: The study was conducted using hole board and passive avoidan...
PHYSICO-MECHANICAL AND FILM-FORMING PROPERTIES OF CARBOXYMETHYL CORN STARCH BUTYRATE
Objective: This study aims to prepare a new hydrophobic, water-soluble modified corn starch via sequential butyrylation and carboxymethylation and to evaluate its physico-mechanical and film-forming properties.Methods: B...
MYRICETIN ISOLATED FROM TURBINARIA ORNATA AMELIORATES ROTENONE INDUCED PARKINSONISM IN DROSOPHILA MELANOGASTER
Objective: Parkinson’s disease (PD) is a neurodegenerative disorder which affects the elderly population. Free radicals overproduction, oxidative stress, apoptosis, inflammation and abnormalities in mitochondria are cr...