ZJXG decoction promoted the fracture healing by reducing the decomposition of BMP-7, IGF-1, bFGF and TGFβ1 in fracture rats
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2014, Vol 5, Issue 1
Abstract
The aim is to investigate the effect of Zhuang Jin Xu Gu (ZJXG) decoction on serum levels of bone morphogenetic protein-7 (BMP-7), insulin-like growth factor-1 (IGF-1), basic fibroblast growth factor (bFGF) and transforming growth factor β1(TGFβ1) and to explore its promoting femoral fracture healing in rats. Femur fractures were generated in 72 male adult Wistar rats by cutting femur transversely at middle point. ZJXG decoction was administered orally after surgery for 28d. The fracture healing process was analyzed by X-ray, gross anatomy and hematoxtlin-eosin (HE) staining. The serum levels of BMP-7, IGF-1, bFGF and TGFβ1 were detected by enzyme linked immunobsorbent assay (ELISA). X-ray imaging and gross anatomy indicated that the fibrous callus tissue at the femoral fracture-end increased and the fracture line became fuzzy at 7-14 d following treatment with ZJXG Decoction. HE staining showed that the fibrous-granular tissue at the fracture-end changed gradually to fibrous, cartilaginous and osseous callus tissues from 7d to 28d. ELISA results showed that the serum levels of BMP-7, IGF-1, bFGF and TGFβ1 increased significantly 28 days following treatment with ZJXG decoction, compared to model group. It is concluded that ZJXG decoction could enhance the fracture healing by reducing the decomposition of BMP-7 and IGF-1, bFGF and TGFβ1 and enhancing their activities.
Authors and Affiliations
Zhen Zhou, Chi Ning, Guanxi Wang, Yunliang Guo, Guangwen Yang
Keywords
Related Articles
- EP ID EP654403
- DOI 10.24297/jab.v5i1.5350
- Views 166
- Downloads 0
DYNAMICAL PROPERTIES OF PLASMID DNA MOLECULE IN PULSE REGIME BY OPTICAL TWEEZERS
The dynamical properties of the plasmid DNA molecule in the optical tweezers using pulsed Gaussian laser beam is investigated. The finite difference Langevin equation (FDLE) describing the dynamics of polystyrene microsp...
Computational prediction & Analysis of Fat mass and Obesity associated genes (FTO) responsible for Childhood obesity
The childhood obesity is raising very critical problem among the children around the globe. More than 40 million children (<5 years) were overweight or obese in 2012 around the globe but it is preventable. Analysis of...
In vitro Rhizogenesis of Pineapple (Ananas comosus L.) Ì Smooth Cayenne Ì Cultivar
This study aimed to develop a rhiyzogenesis protocol for the in vitromicropropagated pineapple plants and subsequently acclimatization the rooted shoots. Two auxins type with different concentrations were tested and seve...
Seasonal variation in the population of Black kite (Milvus migrans), inhabiting the Arid Zone of Rajasthan
The population of Black kite (Milvus migrans) inhabiting rural and urban area of Churu city, Rajasthan was seasonally determined by the direct count method. The counting of the Black kite was made at roosting sites of Ch...
EVALUATION OF THE ANTICANDIDOSIC ACTIVITY OF THE CRUDE AQUEOUS AND ETHANOLIC EXTRACT OF EUCALYPTUS SP, A MYRTACEE FROM THE IVORIAN PHARMACOPEIA ON THE IN VITRO GROWTH OF CANDIDA ALBICANS, CANDIDA GLABRATA and CANDIDA TROPICALIS
With the advent of AIDS/ HIV, we assist to a trong new outbreak of thrive's mycoses. To fight against this mycoses apparition, our research team has tested the whole crude extracts action (water and hydro-alcoholic), out...