ZJXG decoction promoted the fracture healing by reducing the decomposition of BMP-7, IGF-1, bFGF and TGFβ1 in fracture rats
Journal Title: JOURNAL OF ADVANCES IN BIOLOGY - Year 2014, Vol 5, Issue 1
Abstract
The aim is to investigate the effect of Zhuang Jin Xu Gu (ZJXG) decoction on serum levels of bone morphogenetic protein-7 (BMP-7), insulin-like growth factor-1 (IGF-1), basic fibroblast growth factor (bFGF) and transforming growth factor β1(TGFβ1) and to explore its promoting femoral fracture healing in rats. Femur fractures were generated in 72 male adult Wistar rats by cutting femur transversely at middle point. ZJXG decoction was administered orally after surgery for 28d. The fracture healing process was analyzed by X-ray, gross anatomy and hematoxtlin-eosin (HE) staining. The serum levels of BMP-7, IGF-1, bFGF and TGFβ1 were detected by enzyme linked immunobsorbent assay (ELISA). X-ray imaging and gross anatomy indicated that the fibrous callus tissue at the femoral fracture-end increased and the fracture line became fuzzy at 7-14 d following treatment with ZJXG Decoction. HE staining showed that the fibrous-granular tissue at the fracture-end changed gradually to fibrous, cartilaginous and osseous callus tissues from 7d to 28d. ELISA results showed that the serum levels of BMP-7, IGF-1, bFGF and TGFβ1 increased significantly 28 days following treatment with ZJXG decoction, compared to model group. It is concluded that ZJXG decoction could enhance the fracture healing by reducing the decomposition of BMP-7 and IGF-1, bFGF and TGFβ1 and enhancing their activities.
Authors and Affiliations
Zhen Zhou, Chi Ning, Guanxi Wang, Yunliang Guo, Guangwen Yang
Keywords
Related Articles
- EP ID EP654403
- DOI 10.24297/jab.v5i1.5350
- Views 189
- Downloads 0
The effect of jujube oil on some biological activities of Culex pipiens mosquito
Synthetic insecticides are quick, effective and most popular methods of pest control. But because of their unfavorable effects on environment and non-target organisms, there is a growing need to find new, safe effective...
ELEVATION OF β HUMAN CHORIONIC GONADOTROPIN HORMONE AND FIBRINOGEN CONCENTRATION AS AN IMMUNOPROTECTIVE MECHANISM DURING PREGNANCY.
This study was aimed at determining the level of serum β human chorionic gonadotropin hormones and fibrinogen concentration of pregnant women in Benin City. Subjects aged between 18 and 42 years participated in the st...
Assessment of the insecticidal activity of aqueous extracts of two local plants for the management of Leucinodes orbonalis Guenee, (Lepidoptera : Pyralidae ), pest of eggplant (Solanum aethiopicum), in guinean area of Cote d' lvoriee
Leucinodes orbonalis is a major pest of eggplant (Solanum aethiopicum). Control tests with aqueous extracts of two local plants have been carried out on this pest in Azagui located Guinean area of Co´te d'Ivoire. The exp...
Effects of Plant Drugs on Lipid Peroxidation in Irradia ted Cells Onion Seeds
It is shown that seed treatment with herbal preparations before irradiation reduced the level of lipid peroxidation in the cells of Allium cepa.
Testosterone Level, Testicular Histology and Reproductive Events of Caucasian Pit Viper, Gloydius Halys Caucasicus (Serpentes: Viperidae)
The steroid hormones regulate the reproductive physiology of animals and may influence by habitat and seasonal conditions. In this study, body weight and length, testosterone concentration and testicular histology were m...