Bioresearch Communications

Bioresearch Communications

Basic info

  • Publisher: Dhaka University
  • Country of publisher: bangladesh
  • Date added to EuroPub: 2019/Sep/29

Subject and more

  • LCC Subject Category: Biological Sciences
  • Publisher's keywords: Biological science
  • Language of fulltext: english
  • Time from submission to publication: weeks

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  • Article Processing Charges (APCs): No
  • Submission charges: No
  • Waiver policy for charges? Yes

Open access & licensing

  • Type of License:
  • License terms
  • Open Access Statement: Yes
  • Year open access content began: 2015
  • Does the author retain unrestricted copyright? True
  • Does the author retain publishing rights? True

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This journal has '103' articles

EFFECTS OF GERMINATION TIME OF SOYBEAN TO THE ACTIVITY OF SOYBEAN UREASE EZYME

EFFECTS OF GERMINATION TIME OF SOYBEAN TO THE ACTIVITY OF SOYBEAN UREASE EZYME

Authors:
Year: 2019, Volume: 5, Number: 1
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Abstract

The seed germination activity of the enzyme will increase. Urease content in soybeans is relatively high. Investigating the factors affecting the germination process in order to find the time to obtain the highest active enzyme urease. Soybeans were germinated at temperatures (20oC, 24oC, 28oC, 32oC) and humidity (75%, 80%, 85%, 90%, 95%) then surveyed over time. The optimum temperature and humidity for the activity of urease enzyme in soybeans was 28oC, 80% in first day of germination.

Keywords: germination, soybean, enzyme, urease
Corchorus L. and Hibiscus L.: Molecular Phylogeny Helps to Understand Their Relative Evolution and Dispersal Routes

Corchorus L. and Hibiscus L.: Molecular Phylogeny Helps to Understand Their Relative Evolution and Dispersal Routes

Authors:
Year: 2015, Volume: 1, Number: 1
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Abstract

Members of the genera Corchorus L. and Hibiscus L. are excellent sources of natural fibers and becoming much important in recent times due to an increasing concern to make the world greener. The aim of this study has been to describe the molecular phylogenetic relationships among the important members of these two genera as well as to know their relative dispersal throughout the world. Monophyly of Corchorus L. is evident from our study, whereas paraphyletic occurrences have been identified in case of Hibiscus L. Although C. olitorius is assumed to be originated from Africa and C. capsularis from Indian subcontinent, our study found both to be related through maternal inheritance. This prompted us to put forward a hypothesis with archeological support to explain the dispersal routes of migration of these two Corchorus species. Similar migratory occurrences may also be true for Hibiscus L.

Keywords: Corchorus L., Hibiscus L., phylogenetic relationships, dispersal routes.
Oca2 of Schizosaccharomyces pombe Regulates Pol II Transcriptional Termination

Oca2 of Schizosaccharomyces pombe Regulates Pol II Transcriptional Termination

Authors:
Year: 2015, Volume: 1, Number: 1
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Abstract

We describe a Schizosaccharomyces pombe protein kinase, Oca2, which is involved in transcription termination of a subset of genes. Lack of Oca2 results the accumulation of Pol II over termination regions. Oca2 phosphorylates S. pombe homologue of Ctk2, the putative cyclin subunit of fission yeast Ctk1, in vitro. In S. cerevisae, Ctk1 phosphorylates serine 2 of the CTD of RNA polymerase II (Pol II), a modification associated with transcriptional elongation, termination and co-transcriptional premRNA processing. Both, Oca2 and Ctk2 localize predominantly to terminator regions, and in oca2Δ cells Ctk2 localization to terminators is lost. Oca2 mediated recruitment of Ctk2 may be a prerequisite for correct Pol II release from certain termination sites.

Keywords: transcriptional termination, CTD phosphorylation, polyadenylation factors.
Two Cases of Klinefelter Syndrome Identified by Quantitative Fluorescence PCR (QF-PCR) Method

Two Cases of Klinefelter Syndrome Identified by Quantitative Fluorescence PCR (QF-PCR) Method

Authors:
Year: 2015, Volume: 1, Number: 1
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Abstract

Chromosomal aneuploidy is a common cause of genetic abnormality in humans and leading cause of pregnancy loss and congenital birth defects. Pregnancies with chromosomal aneuploidies that survive to term include trisomy 13, 18, and 21 as well as sex chromosome anuploidies with an extra X of Y chromosome. Cytogenetic analysis of metaphase chromosome either by G-banding or fluorescent in situ hybridization (FISH) have been the standard methods for identifying aneuploidies and balanced translocation. However 1-2 weeks are required for the completion of the test as the fetal cells require several days of in vitro culture. Quantitative fluorescence PCR (QF-PCR) offers a suitable alternative for the diagnosis of chromosomal aneuploides thereby reducing the need of cell culture. QF-PCR analysis includes amplification of chromosome specific short tandem repeats (STRs) and non-polymorphic markers followed by capillary electrophoresis. The main advantage of QF-PCR is its speed, accuracy, ease of automation and allow large number of samples to be investigated at a time. This study reports the successful identification of two cases of Klinefelter syndrome from DNA extracted from peripheral blood and amniotic fluid.

Keywords: aneuploidy, autosome, QF-PCR, Klinefelter Syndrome, trisomy
In Silico Computational Prediction of Anti-Breast Cancer Effect of Abruquinones from Abrus precatorius L.

In Silico Computational Prediction of Anti-Breast Cancer Effect of Abruquinones from Abrus precatorius L.

Authors:
Year: 2015, Volume: 1, Number: 1
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Abstract

Knowledge based searching of phytochemicals with potential anti-breast cancer effect from Abrus precatorius (L) with prediction of mechanism of action using computational molecular docking approach was the aim of this investigation. Three abruquinones (A, B and C) were selected upon chemical association network analysis and literature search as candidate ligands, while estriol and genistein were, respectively, considered as positive and negative control. After structural investigation, the chain A of human estrogen receptor beta (ERβ; PDB: 2YLY) was selected as receptor for docking study. Docking was carried out by Molegro Virtual Docker (MVD) and ParDock. Results of the docking studies suggested the favorable binding of abruquinone B and abruquinone C to ERβ-receptor with respect to genistein. Ligand validation was confirmed by the drug-likeness characteristics of abruquinones without any violation of Lipinski's rule. Based on the docking studies it was proposed that anti-breast cancer effect abruquinones might be accomplished by their antagonistic effect on estrogen receptor beta.

Keywords: breast cancer, Abruquinones, molecular docking.
Antioxidant Activity of Some Common Seasonal Fruits of Bangladesh

Antioxidant Activity of Some Common Seasonal Fruits of Bangladesh

Authors:
Year: 2015, Volume: 1, Number: 1
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Abstract

Natural antioxidants are beneficial for health promoting properties due to its ability to neutralize oxygen contain harmful, DNA-damaging radicals. Fruits and vegetables are good sources of antioxidants. In this study, Bangladeshi seasonal fruits wood apple, jujube, hog plum and elephant apple juice were analyzed for total phenolic content (TPC), total flavonoid content (TFC) and DPPH assay, which are the parameters for determining antioxidant capacity. The study suggests wood apple is a rich source of antioxidants whereas hog plum is a poor source. In addition, jujube and elephant apple were found to have the antioxidant amount equivalent to apple. Moreover, wood apple can be used commercially as a stored antioxidant source.

Keywords: Antioxidant, wood apple, jujube, elephant apple, hog plum, total phenolic content (TPC), total flavonoid content (TFC), DPPH assay, IC50.
Genetic Analysis of Y-Chromosome 17 STR in Four Indigenous Populations from Bandarban

Genetic Analysis of Y-Chromosome 17 STR in Four Indigenous Populations from Bandarban

Authors:
Year: 2015, Volume: 1, Number: 1
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Abstract

Despite rapidly growing understandings and dependency on single nucleotide polymorphisms (SNPs), highly variable autosomal 17 Y-chromosome short tandem repeats (STR) are still regarded as the most established method to differentiate individuals. Ethnic and cultural diversity of Bandarban area throughout the Chittagong Hill Tract (CHT) suggests that this hilly range play vital role in genetic evolution of the region. Our previous study suggests that this mountain area acted as a corridor to gene flow across the Indian midland to CHT of Bangladesh. In the present study, we analyzed 17 Ychromosomal short tandem repeat (Y-STR) haplotypes to investigate the Y-STR diversity of four indigenous populations from Bandarban. This study included 55 unrelated male samples from four ethnic populations (Tanchangya, Khumi, Khyang and Mro) were analyzed, among which 41 were unique and 14 Y-STR profiles are shared across the four populations. Khumi and Khyang exhibit relatively high degree of genetic homogeneity lower than 0.5, whereas Tanchangya and Mro represent the other extreme with all loci registering values above 0.5 for the same parameter.

Keywords: CHT indigenous Population, Bandarban, 17 Y-STR, Allelic frequencies, Gene diversity.
Effect of Watering Frequency on Proximate Analysis of Pink Oyster Mushroom

Effect of Watering Frequency on Proximate Analysis of Pink Oyster Mushroom

Authors:
Year: 2015, Volume: 1, Number: 1
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Abstract

Supplemented sawdust with different times of watering has profound effect on chemical composition of Pink Oyster mushroom. Three times watering per days on mushroom had highest amount of carbohydrate (42.42%) and lipid (4.66%) whereas moisture (90.39%), dry matter (10.01%) and crude fiber (21.81%) was highest at watering frequency of four times. Protein content was highest (32.37%) at two times per days of watering. Mineral content of the fruiting body found to be significantly varied on watering frequency. Increasing watering frequency was negatively correlated with the decreasing one. Nitrogen, phosphorus, potassium and magnesium contents were highest when watering frequencies were one to two times per day. But Copper, Manganese and Zinc were obtained at highest levels for the three to four times of watering.

Keywords: Pleurotus djamor, watering frequency, proximate composition.
Computational Approach to Design a Potential siRNA Molecule to Silence the Nucleocapsid Gene of Different Nipah Virus Strains of Bangladesh

Computational Approach to Design a Potential siRNA Molecule to Silence the Nucleocapsid Gene of Different Nipah Virus Strains of Bangladesh

Authors:
Year: 2015, Volume: 1, Number: 1
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Abstract

Nipah virus (NiV) is a single stranded negative-strand RNA virus which was first identified in Malaysia and Singapore. It causes fatal respiratory illness and encephalitis in humans. The progression of this infection is very rapid and causes death within 18 days of infection in most of the cases. Scientists are yet to find out a successful therapeutic approach to treat the NiV affected people. In this study, we have tried to utilize different computational methods to design a siRNA molecule to silence the Nucleocapsid gene of Nipah virus. The nucleocapsid protein (N) gene is one of the most suitable targets for the diagnosis and treatment of NIVs. To find out a common siRNA molecule against five different Nipah virus strains, at first the complete nucleocapsid gene sequences of these five strains were collected from NCBI database. SiDirect 2.0 server was used to design a siRNA molecule against these strains. The siRNA molecule was checked for its secondary structure and GC content using Mfold and OligoCalc server respectively. Blast tool was used to identify any off-target similarity with the designed siRNA molecule. In this study we have proposed a duplex siRNA molecule for N gene silencing of five different strains of NiVs. The siRNA molecule was rationally designed and authenticated using different computational methods. It was also confirmed that the designed siRNA does not match with any off-target sequences. There is no effective therapy available at present to cure Nipah virus infections. The designed siRNA molecule might provide an alternative therapeutic approach against the five major Nipah virus strains in Bangladesh.

Keywords: molecular therapy, therapeutic siRNA, siDirect, Reynolds rule
Evaluation of Antioxidant Status in Beta Thalassemia Major Patients in Sabah, Malaysian Borneo

Evaluation of Antioxidant Status in Beta Thalassemia Major Patients in Sabah, Malaysian Borneo

Authors:
Year: 2015, Volume: 1, Number: 1
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Abstract

In beta thalassemia major severe haemolysis and repeated blood transfusions lead to excess iron deposition in various body tissues. This secondary iron overload is thought to be responsible for peroxidative tissue injury and subsequent oxidative stress. The aim of this study was to determine oxidative stress and serum antioxidant levels in patients with beta thalassemia major. Serum levels of reduced glutathione (GSH), catalase and glutathione S-transferase (GST) as well as lipid peroxides were determined. The Serum GSH, catalase and GST levels in beta thalassemia major patients were found to be 0.30±0.10 (µmol/ml), 5.84±2.17 (nmol/mg protien) and 86.25±15.10 (nmol/mg protein) while in healthy controls they were 1.34±0.29 (µmol/ml), 4.76±0.52 (nmol/mg protein) and 31.97±7.12 (nmol/mg protein) respectively. Serum levels of TBARS in beta thalassemia major patients and in controls were found to be 1.34±0.31 (nmol MDA/ml) and 0.81±0.19 (nmol MDA/ml) respectively. We found marked lower serum GSH levels (p <0.05) while significantly higher levels of serum catalase, GST and TBARS (p<0.05) in patients with beta thalassemia major as compared to healthy controls. Our results suggests that the peroxidative status generated by reactive oxygen species in beta thalassemia major patients may lead to significantly increased production of TBARS which is concomitant with increase in catalase and GST activity.

Keywords: Beta Thalassemia major, reduced Glutathione, Catalase, Glutathione S-transferase, lipid peroxidation, antioxidant enzyme
Pattern of Drug Resistance of Pathogenic Microbes in the Street Foods of Dhaka City, Bangladesh

Pattern of Drug Resistance of Pathogenic Microbes in the Street Foods of Dhaka City, Bangladesh

Authors:
Year: 2015, Volume: 1, Number: 1
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Abstract

The ready-to-eat (RTE) foods sold by the street vendors cause numerous public health hazards. A pilot study was carried out in three areas of Dhaka city, namely Dhanmondi (posh residential area), Lalbag (Low income group residential area) and Motijheel (Commercial area) to assess the microbial load of seven randomly chosen commonly consumed street-vended foods (SVFs) – Bhelpuri, Cake, Cholaboot, Ghugni, Samucha, Singara and Sugarcane juice. The mean aerobic plate count (APC), total coliform (TC) and total Escherichia coli (EC) count ranged from 2.74-3.78, 1.77-2.58 and 1.57-2.64 log10 CFU/g respectively for all the foods tested. According to the guidelines for RTE foods, the APC for Bhelpuri, Cholaboot, Cake and Ghugni is in the satisfactory level whereas for Samucha and Singara it is in the acceptable level. However, the APC, TC and EC count for sugarcane juice are 3.55-4.16, 2.76-3.54 and 2.46-3.39 log10 CFU/ml respectively and all these values fall in the unsatisfactory level. Taking 10 bacterial isolates randomly from each food item of all three areas, a total of 210 colonies were isolated to check the antimicrobial resistant pattern. 79.52% of isolates did not show any antibiotic resistance while the remaining 20.47% of isolates showed resistance to single/multiple antibiotics. Electrophoresis of DNA extract from antibiotic-resistant-isolates showed the presence of plasmid DNA of various sizes. One of the isolate from Bhelpuri of Dhanmondi showed resistance to ampicllin (Amp), neomycin (Neo) and penicillin (Pen) and was identified biochemically as Hafnia alvei. Another isolate from Sugarcane juice of Motijheel showed resistance to Amp, gentamycin (Gen), kanamycin (Kan), Neo and Pen and was identified biochemically as Klebsiella pneumoniae.

Keywords: Street food, Microbes, Antimicrobial resistant bacteria, Dhaka, Bangladesh
Microbiological Analysis of Bangladeshi Paper Currency Circulating in Dhaka City

Microbiological Analysis of Bangladeshi Paper Currency Circulating in Dhaka City

Authors:
Year: 2015, Volume: 1, Number: 1
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Abstract

Paper currency notes which are transferred from one individual to other are known to carry bacteria on their surface and are responsible for transmitting them to human. The present study was thus conducted to evaluate the present status of Bangladeshi paper currency concerning the bacterial contamination. A total of 300 different valued banknotes were obtained from 10 different occupational persons. Significantly higher bacterial concentration was detected in bank notes sampled from hawker than that of other sources while significantly lower count was found from students (p<0.05). However, similar total bacterial count was observed in bank notes obtained from fish seller and fruit seller. Similar density of Staphylococcus aureus, Staphylococcus spp. Streptococcus spp. were found in bank notes sampled from all sources. Escherichia coli, Klebshialla, Proteus and Enterobacter, Salmonella and Shigella were isolated from different bank notes of different. Significantly higher concentration of V. cholera and other vibrios were detected in bank notes sampled from fish seller than those of other sources. Similar concentration of Psedomonas, Alcaloigens, Proteus using pseudomonas agar media was also detected in currencies sampled from all sources. However, significantly higher fungal density was detected in currencies obtained from beggar and fruit seller while lower concentration was observed in bank notes sampled from food seller (p<0.05). A total of 60 bacterial strains were isolated from 10 different sources and from 6 different currencies. Thus findings of the present study suggest that paper currency in circulation in Bangladesh may act as the reservoir of potentially pathogenic bacteria.

Keywords: paper currency, banknotes, bacterial contamination, slum dwellers, bus conductor.
Fatty Acid Binding Protein 3 (FABP3) Deficiency Does Not Impact on Feto-Placental Morphometry and Fatty Acid Transporters in Mice

Fatty Acid Binding Protein 3 (FABP3) Deficiency Does Not Impact on Feto-Placental Morphometry and Fatty Acid Transporters in Mice

Authors:
Year: 2015, Volume: 1, Number: 2
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Abstract

In most mammals, proper placentation along with its morphology is critical for fetal development and pregnancy success. Fatty acid binding protein 3 (FABP3) has been shown to regulate the transportation of polyunsaturated fatty acid (PUFA) to the fetus, but its impact on placental morphology and genetic alteration of fatty acid transporters in trophoblast cells is still unknown. Using mice model we found Fabp3 deficiency does not affect the morphology of placenta along with the gene of fatty acid transporters. FABP3 deficiency was also found to have no impact on the morphology of human trophoblast cells (BeWo cells) in in vitro study. Fatty acid transporter genes were also remains unaltered after FABP3 knockdown. Our study suggests that placenta may conserve its morphology and genetics although it has PUFAs deficiency.

Keywords: FABP3, PUFA, placenta, trophoblast, morphometry
Significant Association of ADAM33 V4C>G Polymorphisms with Asthma in a North Indian Population

Significant Association of ADAM33 V4C>G Polymorphisms with Asthma in a North Indian Population

Authors:
Year: 2015, Volume: 1, Number: 2
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Abstract

ADAM33 is zinc-dependent metalloproteases comprised of seven domains and has range of single nucleotide polymorphisms. These polymorphisms were found to be genetically associated in bronchial hyperresponsiveness that accounts its involvement in asthma. A case-control study was conducted with 483 healthy controls and 481 asthma patients in the present study. DNA samples were extracted from blood and the genotyping was done using PCR-RFLP method. Statistical analysis revealed that ADAM33 V4C>G polymorphism show highly significant association towards asthma with significant OR=1.40, p=0.017 in CG genotype and two fold risk in mutant GG genotype (p=0.000). Furthermore, mutant allele G was also highly significant towards the disease with OR=1.39 and p=0.000. However, ADAM33 F+1G>A, rs511898 gene polymorphism was significantly associated with asthma in only male and female phenotypes. This study concludes that the ADAM33 gene polymorphism plays an important role in the pathogenesis of asthma in a North Indian population.

Keywords: ADAM33, asthma, case-control study, polymorphism, PCR-RFLP, total IgE
Cloning and Characterization of a Tissue Specific Promoter GluB-1 from Nipponbare by Transformation in Rice

Cloning and Characterization of a Tissue Specific Promoter GluB-1 from Nipponbare by Transformation in Rice

Authors:
Year: 2015, Volume: 1, Number: 2
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Abstract

Tools of plant biotechnology can be applied as a routine procedure for improving rice grain quality or production of desired recombinant proteins in the rice seeds. For this purpose, endosperm specific promoter is a useful choice as desired gene expression would take place only in rice seed and not in root or shoot. Also, rice seed has become an attractive bioreactor for the production of recombinant proteins compared to other cereals. Glutelins are the most abundant storage proteins in rice grain which constitute up to 80% of the total protein content. The promoter region of GluB-1, one of the glutelin genes in rice, has been used as a model to study the regulation of seed-storage protein accumulation. In this study the upstream region (~2.4 kb) of the GluB-1 gene was amplified from the genomic DNA of the Nipponbare cultivar of Oryza sativa (japonica group) and then cloned successively into an entry and promoter‐characterization binary destination vector having the reporter gene β‐glucuronidase (GUS) by applying Gateway Technology. Three plants generated by Agrobacterium mediated tissue culture were confirmed by PCR with GluB-1 specific primer. In the transformed plants, histochemical GUS assay showed no expression in the root and shoot but was prominent in the endosperm of the T1 seeds. Therefore this 2.4 kb promoter from Nipponbare rice can be successfully used to improve rice grain quality or express stable recombinant proteins in rice seeds for therapeutic purposes.

Keywords: glutelin, Nipponbare cultivar, β‐glucuronidase (GUS), Agrobacterium-mediated transformation

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