基因组消减杂交技术筛选肾透明细胞癌转移相关甲基化基因
Journal Title: Di-er Junyi Daxue Xuebao - Year 2008, Vol 29, Issue 5
Abstract
目的：筛选能够早期诊断和预测肾透明细胞癌转移相关基因的甲基化生物标志。方法：应用本室从临床新鲜组织标本体外培养建立的低转移性和高转移性肾透明细胞癌细胞株，病理学鉴定后提取基因组DNA，应用3轮NotⅠ基因组消减杂交的方法富集差异甲基化序列，差异序列连接载体，转化宿主菌和α筛选后，选取40个序列进行测序，应用生物信息学分析确定启动子CpG岛甲基化序列，并对未知基因功能进行预测。结果：DNA测序后获得了27个单一克隆在高转移和低转移性肾透明细胞癌基因组中存在甲基化差异；其中5个序列具有CpG岛，其中2个对应于基因MYADM和LOC646024的启动子区。MYADM与血细胞成熟、干细胞再生有关；而LOC646024与NK细胞抗肿瘤相关的UL16结合蛋白1有85%的相似度。结论：本研究从不同转移能力的中国人肾透明细胞癌基因组中获得多种转移相关性的新甲基化序列，发现MYADM和LOC646024候选基因与肾癌转移相关。
Authors and Affiliations
Jun-wen SUN, Wen-jun CHANG, Yu-jia ZHAI, Xiao-jie TAN, Jian-guo HOU, Yong-wei YU, Li-ye MA, Guang-wen CAO
Keywords
Related Articles
- EP ID EP82850
- DOI -
- Views 152
- Downloads 0
Low-dose naloxone combined with sufentanil and ropivacaine for postoperative patient-controlled epidural analgesia in elderly patients undergoing total hip replacement
Objective:To evaluate the effect and safety of low-dose naloxone combined with sufentanil and ropivacaine for postoperative patient-controlled epidural analgesia (PCEA) in elderly patients undergoing total hip replacemen...
Retroperitoneal laparoscopic partial nephrectomy for early malignant renal tumors: a report of 21 cases <br /> <br />
Objective：To explore the procedures and clinical outcomes of retroperitoneal partial nephrectomy for early malignant renal tumors. Methods: A total of 21 patients (19 men and 2 women, with a mean age of 49 years) with e...
Assessing mandibular bone mineral density with morphometric analysis
Objective：To determine the effectiveness and validity of morphometric analysis made from dental panoramic tomograms (DPTs) for predicting mandibular bone mineral density (BMD) while taking the result of dual energy Xra...
重组质粒pSUPER-HBs RNAi的构建及筛选
目的:利用pSUPER-RNAi载体系统进行HBs RNAi序列的初步筛选和干扰效果鉴定。方法:设计并合成针对HBs基因区的3条siRNA(HBs-siRNA1、HBs-siRNA2、HBs-siRNA3),构建3种重组载体pSUPER-HBs-siRNA,与HBV质粒同时瞬转人胚肾293T细胞,72 h后观察...
Needle-tract bleeding after percutaneous radiofrequency ablation for hepatocellular carcinomas: risk factors,prevention and treatment
Objective:To analyze the risk factors,prevention and treatment of needle-tract bleeding after percutaneous image-guided radiofrequency ablation (PRFA) for hepatocellular carcinomas (HCC).Methods: A total of 1 567 RFA ses...