抗PIWIL2蛋白多克隆抗体的制备、鉴定及初步应用
Journal Title: Di-er Junyi Daxue Xuebao - Year 2008, Vol 29, Issue 9
Abstract
目的:制备兔抗人PIWIL2(P-element-induced wimpy testis like 2,或称HIWI2)的多克隆抗体,并鉴定其特异性,初步探讨其在人正常及肿瘤组织中的分布。方法:人工合成特异性PIWIL2多肽,以马来酰胺活化匙孔血蓝蛋白(Keyhole limpet hemocyanin,KLH)为载体构建多肽免疫原,致敏大白兔,制备兔抗人PIWIL2多克隆抗体。亲和层析法纯化抗体,ELISA和Western印迹鉴定特异性,人组织芯片行PIWIL2的免疫组化研究。 结果:通过构建PIWIL2多肽-KLH载体复合物致敏大白兔,制备出兔抗人PIWIL2蛋白多克隆抗体。ELISA及Western印迹证实兔抗人PIWIL2抗体可特异性识别PIWIL2多肽。人组织芯片免疫组化染色显示,PIWIL2在肿瘤细胞的表达具有组织特异性,大多数正常和癌组织的平滑肌细胞胞质中呈阳性染色。 结论:成功制备出兔抗人PIWIL2多克隆抗体,为后续研究奠定了基础。
Authors and Affiliations
Feng-shang ZHU
Keywords
Related Articles
- EP ID EP84207
- DOI -
- Views 116
- Downloads 0
99mTc-MIBI myocardial perfusion image in predicting therapeutic effect of percutaneous coronary intervention
Objective：To observe the 99mTc-MIBI myocardial perfusion images in patients with myocardial infarction before/after percutaneous coronary intervention (PCI),so as to screen for the reliable predictors for the therapeuti...
驻极体透皮贴剂体外实验的优化设计探讨
目的:目前驻极体透皮贴剂的体外实验多采用比较低效的单因素实验设计方法。为了更系统和有效地分析各因素之间的影响,本文根据驻极体透皮贴剂的研究目标,提出驻极体透皮贴剂的体外实验的优化方案。方法:由于影响驻极体电荷储存稳定性的因素...
Explant combined with trypsin-digestion for culture of endothelial cells from infantile hemangiomas
Objective:To explore a novel method for cultivation of the endothelial cells(EC) from infantile hemangiomas(IH) in vitro and observe the biological character of cultured endothelial cells.Methods:Fresh operative specimen...
Needle-tract bleeding after percutaneous radiofrequency ablation for hepatocellular carcinomas: risk factors,prevention and treatment
Objective:To analyze the risk factors,prevention and treatment of needle-tract bleeding after percutaneous image-guided radiofrequency ablation (PRFA) for hepatocellular carcinomas (HCC).Methods: A total of 1 567 RFA ses...
结核杆菌抗原Ag85A基因的克隆及在大肠杆菌中的表达
目的:将结核分枝杆菌Ag85A抗原基因克隆并在大肠杆菌中表达。方法:用PCR方法从结核分枝杆菌基因组中扩增Ag85A抗原基因,然后将其插入到原核表达载体pGEX5T中,构建成pGEX5TAg85A重组质粒。经IPTG诱导使该基因在E.coli k802菌中表达,亲和层...