抗PIWIL2蛋白多克隆抗体的制备、鉴定及初步应用
Journal Title: Di-er Junyi Daxue Xuebao - Year 2008, Vol 29, Issue 9
Abstract
目的:制备兔抗人PIWIL2(P-element-induced wimpy testis like 2,或称HIWI2)的多克隆抗体,并鉴定其特异性,初步探讨其在人正常及肿瘤组织中的分布。方法:人工合成特异性PIWIL2多肽,以马来酰胺活化匙孔血蓝蛋白(Keyhole limpet hemocyanin,KLH)为载体构建多肽免疫原,致敏大白兔,制备兔抗人PIWIL2多克隆抗体。亲和层析法纯化抗体,ELISA和Western印迹鉴定特异性,人组织芯片行PIWIL2的免疫组化研究。 结果:通过构建PIWIL2多肽-KLH载体复合物致敏大白兔,制备出兔抗人PIWIL2蛋白多克隆抗体。ELISA及Western印迹证实兔抗人PIWIL2抗体可特异性识别PIWIL2多肽。人组织芯片免疫组化染色显示,PIWIL2在肿瘤细胞的表达具有组织特异性,大多数正常和癌组织的平滑肌细胞胞质中呈阳性染色。 结论:成功制备出兔抗人PIWIL2多克隆抗体,为后续研究奠定了基础。
Authors and Affiliations
Feng-shang ZHU
Keywords
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