Changes of P2X3 receptor expression in dorsal root ganglia in a rat model of bone cancer pain
Journal Title: Di-er Junyi Daxue Xuebao - Year 2009, Vol 30, Issue 3
Abstract
Objective:To observe the immunoreactive changes of P2X3 receptor and examine P2X3 receptor mRNA level in the dorsal root ganglia (DRG) in a rat model of bone cancer pain. Methods: Sixty female Wistar rats, weighing 150-200 g, were randomly allocated to three groups (n=20 each): group CP (cancer pain) received intra-tibial injection of Syngeneic Walker 256 mammary gland carcinoma cells (2×105 ), group S received intra-tibial injection of equal volume 0.9% sodium chloride, and group C received blank control. Mechanical allodynia and thermal hyperalgesia tests were taken on 4, 7, 10, 14, 17, and 21 days after inoculation. On day 14 and 21, ten rats of each group were chosen for either immunohistochemistry or real-time RT-PCR to examine the changes of P2X3 receptor and mRNA. Results: The rats in group CP began to display hyperalgesia on day 10 and the pain became most obvious between day 14 and 21. Immunohistochemistry showed that the proportion of DRG neurons positive of P2X3 receptor in group CP was significantly increased on 14 and 21 d(P<0.05), and RT-PCR showed that the expression of P2X3 receptor mRNA was also significantly increased. Conclusion: These results suggest that our rat model of bone cancer pain has hyperalgesia, which might be related to the increase of P2X3 receptor expression.
Authors and Affiliations
Jing-xiang WU, Mei-ying XU, Xue-rong MIAO, Xiao-qing LI, Wei-feng YU
Keywords
Related Articles
- EP ID EP83060
- DOI -
- Views 120
- Downloads 0
Expression of DNA methyltransferase 1 and histone deacetylase 1 proteins in intraductal papillary mucinous neoplasms
Objective:To investigate the expression of DNA methylation transferase 1 (DNMT1) and histone deacetylase 1 (HDAC1) proteins in intraductal papillary mucinous neoplasms (IMPNs) and its possible clinical significance.Metho...
HPLC法同时测定中药丹参中水溶性和脂溶性成分的含量
目的:建立同时测定丹参中2种水溶性成分和2种脂溶性成分的方法。方法:HPLC-DAD法,采用Agilent Zorbax TC C18柱(4.6 mm×250 mm,5 μm),以甲醇2%醋酸为流动相进行梯度洗脱:0～15 min,30% B～40% B; 15～20 min,40% B～60% B; 20～25 min,60%...
Take full advantage of military medical university to upgrade the military health service capability
The rapid medical rescue action by People’s Liberation Army after Wenchuan Earthquake demonstrated the great role of military health rescue power; meanwhile, it also uncovered some problems in the military health servic...
The development of quorum sensing phenomenon in pathogenic fungi
The quorum sensing commonly exists in procaryote kingdom, regulating various biologic functions. Recently similar phenomenon was also found in fungi world, which affecting both biofilm formation and dimorphism. In this a...
NPTX2甲基化在胰腺癌中的定量检测及诊断应用
目的:检测NPTX2甲基化水平在胰腺癌中变化并评价其用于胰腺癌诊断的价值。方法:在10例胰腺癌组织和10例癌旁正常胰腺组织中,分别应用定量RT-PCR和SYBR Green荧光掺入甲基化特异性定量检测法检测NPTX2 mRNA表达量及CpG岛的甲基化量。应用上...