NPTX2甲基化在胰腺癌中的定量检测及诊断应用
Journal Title: Di-er Junyi Daxue Xuebao - Year 2008, Vol 29, Issue 11
Abstract
目的:检测NPTX2甲基化水平在胰腺癌中变化并评价其用于胰腺癌诊断的价值。方法:在10例胰腺癌组织和10例癌旁正常胰腺组织中,分别应用定量RT-PCR和SYBR Green荧光掺入甲基化特异性定量检测法检测NPTX2 mRNA表达量及CpG岛的甲基化量。应用上述建立甲基化特异性定量检测方法分析NPTX2甲基化在胰腺癌及健康志愿者全血DNA中的差异。结果:NPTX2基因在胰腺癌组织中mRNA表达量低于癌旁正常胰腺组织 (RQ,0.276±0.263 vs 3.526±3.037,P＝0.001),而其甲基化指数(MI)较癌旁正常胰腺组织高[(9.02±7.52)% vs (1.28±0.98)%,P＝0.003]。RQ与MI两者之间存在负相关关系(R=-0.552,P=0.012)。NPTX2基因MI在胰腺癌患者全血DNA中显著高于健康人[(1.80±1.76)% vs (0.84±0.45)%,P<0.05]。结论:在胰腺癌组织中NPTX2发生高甲基化变化,检测其在临床样品中的MI可辅助用于胰腺癌诊断。
Authors and Affiliations
Jia CAO
Keywords
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