Construction of PSAspecific dendritic cell vaccine and its in vitro immune activity

Journal Title: Di-er Junyi Daxue Xuebao - Year 2009, Vol 30, Issue 3

Abstract

Objective:To construct of PSAspecific dendritic cell (DC) vaccine and to observe its in vitro antitumour activity, so as to pave a way for future study. Methods: Bone marrow precursors were isolated and bone marrow derived DCs were prepared. Mature DCs were pulsed by PSA, Lysate of cancer cells, OVA and PBS to yield PSADC, Lys DC, OvaDC, and NonDC, respectively. After primed by antigen, the changes of IL12 p70 and IL1β in the supernatant of dendritic cells were assessed by ELISA. The antigenspecific proliferation and cytotoxicity activity of T cellprimed by PSApulsed DCs were observed and the results were compared with those by Lys, Ova and PBSpulsed DCs. Results: Mature DCs were successfully derived from bone marrow cells with a purity higher than 95%. ELISA assay showed PSADC, LysDC and OvaDC group secreted high levels of IL12 p70 and IL1β than NonDC group (P<0.05). In addition, PSADCs and LysDCs had significantly stronger ability to stimulate the proliferation of CD4+T cells in 3day classic mixed lymphocyte reaction (MLR) compared with OvaDCs and NonDCs (P<0.01). Higher levels of IFNγ and IL2 were detected in PSADCs and LysDCs groups compared with the other two groups (P<0.01), whereas the levels of IL10 and IL4 were lower than the other two groups (P<0.05). Moreover, PSADCs and Lys DCs enhanced DTH responses of C57BL/6 mice after antigen immunization; the third antigen and control did not show the enhancement effect (P<0.05). To observe the in vitro antiPSA CTL reactions induced by PSADCs and LysDCs, the LNCaP cell line (PSA specific) was used as syngeneic target and the E.G7 cell line (H2b) was used as Ovaspecific target cells. Compared with OvaDCs and NonDCs, CTL cells induced by PSADCs, LysDCs had significantly enhanced antigenspecific CTL activity to LNCaP cells (P<0.05).Conclusion: DCbased PSAepitope vaccine can be prepared by pulsing DCs with PSA protein; the prepared vaccine has strong in vitro immune activity and can kill LNCaP cells.

Authors and Affiliations

Dan feng XU, Yi GAO, Yu shan LIU, Xin gang CUI, Jian ping CHE, Lei YIN, Ji zhang XING, Ya cheng YAO, Ji zhong REN, Zhi lian MIN

Keywords

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  • EP ID EP84309
  • DOI -
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How To Cite

Dan feng XU, Yi GAO, Yu shan LIU, Xin gang CUI, Jian ping CHE, Lei YIN, Ji zhang XING, Ya cheng YAO, Ji zhong REN, Zhi lian MIN (2009). Construction of PSAspecific dendritic cell vaccine and its in vitro immune activity . Di-er Junyi Daxue Xuebao, 30(3), 260-264. https://europub.co.uk/articles/-A-84309