Objective:To clone human PKGⅠα gene and construct a recombinant adenovirus vector containing wild-type PKGⅠα.Methods: RT-PCR was used to amplify the full-length PKGⅠα gene from human pulmonary arterial smooth muscle.Afte...
Objective:To investigate the anti-inflammatory and analgesic activity of Eucommiae ulmoides seed total glycoside(EUG). Methods: Dimethyl benzene(DB)-induced mouse ear edema model and carrageein-induced rat paw edema mode...
Construction and identification of recombinant adenovirus containing wild-type human PKGⅠα gene
Objective:To clone human PKGⅠα gene and construct a recombinant adenovirus vector containing wild-type PKGⅠα.Methods: RT-PCR was used to amplify the full-length PKGⅠα gene from human pulmonary arterial smooth muscle.Afte...
基因组消减杂交技术筛选肾透明细胞癌转移相关甲基化基因
目的:筛选能够早期诊断和预测肾透明细胞癌转移相关基因的甲基化生物标志。方法:应用本室从临床新鲜组织标本体外培养建立的低转移性和高转移性肾透明细胞癌细胞株,病理学鉴定后提取基因组DNA,应用3轮NotⅠ基因组消减杂交的方法富集差...
减毒沙门菌介导的TRAIL和VP3对胃癌细胞的生长抑制作用及其机制
目的:利用沙门菌作为真核质粒携带载体,在体内外观察肿瘤坏死因子相关凋亡诱导配体(TRAIL)和鸡贫血病毒VP3基因对胃癌细胞的生长抑制作用。方法:将重组质粒pBud-TRAIL、 pBud-VP3、 pBud-TRAIL-VP3电转化减毒沙门菌SL7207,经稳定性鉴定后...
Anti-inflammatory and analgesic activity of Eucommiae ulmoides seed total glycoside
Objective:To investigate the anti-inflammatory and analgesic activity of Eucommiae ulmoides seed total glycoside(EUG). Methods: Dimethyl benzene(DB)-induced mouse ear edema model and carrageein-induced rat paw edema mode...
抗PIWIL2蛋白多克隆抗体的制备、鉴定及初步应用
目的:制备兔抗人PIWIL2(P-element-induced wimpy testis like 2,或称HIWI2)的多克隆抗体,并鉴定其特异性,初步探讨其在人正常及肿瘤组织中的分布。方法:人工合成特异性PIWIL2多肽,以马来酰胺活化匙孔血蓝蛋白(Keyhole limpet hemocyanin,K...