Antimicrobial activity and biosynthesis of nanoparticles by endophytic bacterium inhabiting Coffee arabica L.
Journal Title: Scientific Journal of Biological Sciences - Year 2012, Vol 1, Issue 5
Abstract
The interface between endophytes and nanomaterials is a relatively new and unexplored area the present study evaluates screening of bacterial endophytes from surfaced sterilized leaf and stem segments of agro economical plant Coffee arabica L. towards synthesis of silver nanoparticles and antimicrobial metabolites. Among thirty two endophytes isolated nine isolates exhibited antimicrobial activity among which one bacterium was capable of extracellular synthesis of silver nanoparticles upon evaluation of supernatant with 1 mM of silver nitrate, biosynthesis of silver nanoparticles were assessed by UV-Visible Spectroscopy and the bacterium was capable of secreting antimicrobial secondary metabolites upon crude ethyl acetate extract evaluated for antimicrobial activity against panel of both gram positive and gram negative as well as phytopathogenic fungi. Partial characterization was carried out via bioautographic technique with Rf value 0.3 and 0.6 exhibiting antimicrobial activity against MRSA strain. Further studies in this area will be promising enough for molecular characterization of endophytic bacterium and chemical profiling of antimicrobial metabolites at the same time physiochemical characterization of nanoparticles will be valuable to reveal the size and shape.
Authors and Affiliations
S. Baker*| Herbal Drug Technological Laboratory, Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India., S. Shreedharmurthy| Herbal Drug Technological Laboratory, Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysore 570 006, Karnataka, India.
Keywords
Related Articles
- EP ID EP1070
- DOI -
- Views 583
- Downloads 42
Effect of temperature and PH on activity of purified cellulase obtained from Aspergillus flavus
In view of the fact that temperature and pH play key role inthe activity of fungal cellulases, this experiment was designed to assess theeffect of temperature, and pH on activity of the purified enzyme fromAspergillus fl...
Haematologic and Biochemical Indices of Plasmodium falciparum Infected Inhabitants of Owerri, Imo State, Nigeria
The present study seeks to investigate changesin haematologic and biochemical indices of moderately P. falciparum infectedmale inhabitants of Owerri Municipality. Haematologic and biochemical indiceswere estimated by spe...
Study of genetic variation and phenetic relationships in some vulnerable taxa from Indian sundarbans following RAPD marker analysis
The mangrove ecosystem plays an important role in maintaining coastal ecological balance and is also one of the most highly productive and dynamic ecosystems to mankind, providing food, livelihood and ecological security...
Cockroaches (periplaneta americana and blattella germanica): reservoirs of multi drug resistant (MDR) bacteria in Uyo, Akwa Ibom State
Bacteriological examination of external surfaces and internal guts of Periplaneta americana and Blattela germanica were carried out using standard method and antibiotics susceptibility profiles of the isolates were deter...
Genetic correlation between egg quality traits
Genetic correlation coefficients between egg quality traits were studiedusing fifty- six harco pullets raised under standard management practices. Theestimates were carried out at 22-week, 26-week, 30-week and 32-week of...