Effect of temperature and PH on activity of purified cellulase obtained from Aspergillus flavus

Journal Title: Scientific Journal of Biological Sciences - Year 2014, Vol 3, Issue 6

Abstract

In view of the fact that temperature and pH play key role inthe activity of fungal cellulases, this experiment was designed to assess theeffect of temperature, and pH on activity of the purified enzyme fromAspergillus flavus derived from rotten wood. The characterizationof the purified cellulase enzyme of Aspergillus flavus was carried out usingcarboxymethyl cellulose, filter paper and cotton wool assays. The maximum andoptimum temperatures were 900C and 700C respectively. The pH optima werebetween 5 and 8. The enzyme retained above 50% of its original activity at 1000C for 1h and up to 80% at 80 0C for 1h. It was stable over a pH range of 5-8,and retained up to 40% of its original activity at pH 9. The study carried outshowed that the purified Aspergillus flavus cellulases demonstrated usefulproperties among which include; high optimum temperature, increased thermalstability at both acidic and alkaline pH. They also offer additional advantageas they are more rigid than meso enzymes and moreover, are more resistant tothermal and chemical denaturation. Hence are remarkable tools for developingcommercial biotechnologies and for studying protein stability.

Authors and Affiliations

I. F. Okonkwo| Department of Agricultural and Bioresources Engineering, Nnamdi Azikiwe University, PMB 5025 Awka, Anambra State, Nigeria.

Keywords

Related Articles

Genetic correlation between egg quality traits

Genetic correlation coefficients between egg quality traits were studiedusing fifty- six harco pullets raised under standard management practices. Theestimates were carried out at 22-week, 26-week, 30-week and 32-week of...

Impact of bacterial and viral infections for barrennes of productive animals

Historically the operational stages of artificial insemination of animals. The influence of some viral and bacterial infections on the reproductive systems in male animals has led to barreness.

Abiotic factors and anti-reproductive action of bait containing eugenol against Lymnaea acuminata

The effect of abiotic factor (temperature, pH, dissolved oxygen, carbon dioxide) on the reproduction of snail L. acuminata fed to bait containing 40% and 80% of 24h LC50 eugenol have been reported in this paper. Feeding...

Effect of temperature and PH on activity of purified cellulase obtained from Aspergillus flavus

In view of the fact that temperature and pH play key role inthe activity of fungal cellulases, this experiment was designed to assess theeffect of temperature, and pH on activity of the purified enzyme fromAspergillus fl...

Irregular cytokinesis during microsporogenesis of psychotria myriantha Mull. Arg.

The Rubiaceae family is composed of approximately 550 generaand 9000 species. In spite of its great number of species and importance, fewcytological studies on the genus Psychotria are extant, whereas cytogeneticanalyses...

Download PDF file
  • EP ID EP1105
  • DOI 10.14196/sjbs.v3i6.1535
  • Views 319
  • Downloads 26

How To Cite

I. F. Okonkwo (2014). Effect of temperature and PH on activity of purified cellulase obtained from Aspergillus flavus. Scientific Journal of Biological Sciences, 3(6), 47-58. https://europub.co.uk/articles/-A-1105