2-Chloro-N-(2-6-Diphenyl) Acetamide Induced Imbalance Of Serum Cortisol Level Correlated With Histopathological Alterations On The Spleen Cells Of Air Breathing Fish Clarias Batrachus(Linn.) : As Bioindicators Of Stress
Journal Title: International Journal of Pharmaceutical Science Invention - Year 2018, Vol 7, Issue 2
Abstract
The present study was carried out to investigate physiological stress induced by Butachlor on serum cortisol level and histopathological alterations in spleen cells of air breathing fish Clarias batrachus(Linn.). Fishes were collected from different wetlands of North Bihar and acclimatized for fifteen days in the Laboratory. The LC50 of Butachlor (EC 35%) was calculated as per standard APHA method, the fishes were then exposed to sub lethal concentration of (1.5µl/L) for a period of 5, 10 and 15 days. After termination of each exposure period, blood was collected from control and pesticide exposed fish, serum was separated and then fishes were sacrificed. The results showed a significant increase in serum cortisol level. A steep decline was observed in the mean value of cortisol level (198.833±1.471) IU on 5 day of exposure as compared to the control fish (201.33 ±5.0) IU. Again on 10th day there was sharp increase (393.5±1.048) IU and after 15th day it showed more than two fold increase (4017.5±1.87) IU than controlled fish. Light photomicrographs of spleen cells showed massive damage to red pulp and white pulp and numerous melanomacrophage centers. Spleen cell also showed vacuolations and necrosis along with fibrosis, and widening of trabaculae.
Authors and Affiliations
Prakriti Verma, Pushpa Kumari, Rita Gupta, S. K. Srivastava
Keywords
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