28-HOMOCASTASTERONE: A NOVEL DIETARY PHYTO KETO OXYSTEROL MODULATING TESTICULAR STEROID METABOLISM AND LxR mRNA EXPRESSION IN DIABETIC RAT
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2018, Vol 10, Issue 2
Abstract
Objective: Present study aims to investigate 28-homocastasterone (28-HC) influences on testicular tissue in the normal and diabetic rat.Methods: Induction of diabetes was achieved by single peritoneal injection of streptozotocin (60 mg/kg b. wt) followed by 28-HC (100 µg/150 gm body weight) administration by oral gavage for 15 consecutive days to experimental rats. 3β-hydroxysteroid dehydrogenase, 17β-hydroxysteroid dehydrogenase activities, testosterone level, Liver X Receptor (LxR) mRNA expression, malondialdehyde (MDA), reduced glutathione (GSH) and testis histology was analysed.Results: Increased cholesterol level, 3β-hydroxysteroid dehydrogenase (3βHSD), 17β-hydroxysteroid dehydrogenase (17βHSD) activities, testosterone level with significant elevation of LxR-α and β mRNA expression in treated rat testis. A significant reduction found inMDA and increased reducedGSH along with improved testicular architecture was observed.Conclusion: In the present study demonstrated that 28-HC induced 3βHSD, 17βHSD enzyme activity and testosterone level, thus indicative of steroidogenic potential and capable of transactivating LxR-α and β molecular operative in rat testicular tissue.
Authors and Affiliations
Velan Athithan, Ramasamy Ramesh, Kotteazeth Srikumar
Keywords
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- EP ID EP575384
- DOI 10.22159/ijpps.2018v10i2.23779
- Views 83
- Downloads 0
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