3,3′-Dimethoxybenzidine. Determination in workplace air
Journal Title: Podstawy i Metody Oceny Środowiska Pracy - Year 2019, Vol 35, Issue 2
Abstract
3,3’-Dimethoxybenzidine (DMOB) is a substance classified as a carcinogen. The recommended maximum admissible concentration (MAC) value for this substance in workplace air is 0.2 mg/m3. The aim of this study was to develop and validate a sensitive method for the determination of 3,3’-dimethoxybenzidine concentrations in workplace air in the range from 1/10 to 2 MAC values, in accordance with the requirements of standard PN-EN 482. The method consists in passing air that contains DMOB through a sulfuric acid-treated glass fiber filters, washing out the substance settled on the filter, using water and solution of sodium hydroxide, liquid-liquid extraction with toluene, replacing dissolvent with acetonitrile and analyzing the obtained solution. Studies were performed using high-performance liquid chromatography (HPLC) technique. An Agilent Technologies (Germany) liquid chromatograph, series 1200, with a fluorescence detector (FLD) was used in the experiment. In the test, an Ultra C18 column of dimensions: 250 x 4.6 mm, with a 10 x 4.0 mm precolumn (Restek, USA) was applied. This method is linear within the 1.08 μg/ml to 21.60 μg/ml working range, which is equivalent to air concentrations from 0.02 to 0.4 mg/m3 for a 54-L air sample. The analytical method described in this paper allows for selective determination of 3,3’-dimethoxybenzidine in workplace air in the presence of 1,4-phenylenediamine, benzidine, aniline, 3,3’-dimethylbenzidine, 2-nitrotoluene, 3,3’-dichlorobenzidine and azobenzene. The method is characterized by good precision and accuracy and meets the criteria for the performance of procedures for the measurement of chemical agents, listed in EN 482. The method may be used for the assessment of occupational exposure to 3,3’-dimethoxybenzidine and the associated risk to workers’ health. The developed method of determining 3,3’-dimethoxybenzidine has been recorded as an analytical procedure (see appendix). This article discusses the problems of occupational safety and health, which are covered by health sciences and environmental engineering.<br/><br/>
Authors and Affiliations
Joanna Kowalska
Keywords
Related Articles
- EP ID EP599824
- DOI 10.5604/01.3001.0013.2527
- Views 99
- Downloads 0
Phthalic anhydride. Determination in workplace air
Phthalic anhydride is a crystalline solid. Phthalic anhydride is an important intermediate in thechemical industry. It is harmful, irritating and sensitizing.The aim of this study was to develop a new method for determin...
[sup][/sup]N,N-Dimethylacetamide. Determining in workplace air with GC/FID
N,N-Dimethylacetamide (DMAC) is a colorless liquid with a characteristic amine odour.N,N-Dimethylacetamide mixes with polar and non-polar organic solvents and water. Thissubstance is used in plastic and fi bers industrie...
2-Ethylhexan-1-ol. Documentation of proposed values of occupational exposure limits (OELs)
2-Ethylhexan-1-ol is a colorless liquid with sweet taste and light, floral, rose-like odour. This aliphatic alcohol is an important intermediate for synthesis of low-volatile esters, e.g., di(2-ethylhexyl) phthalate (DEH...
Oznaczanie bromoetanu w powietrzu środowiska pracy metodą chromatografii gazowej z detekcją MAS
W wyniku przeprowadzonych badań opracowano metodę oznaczania bromoetanu w powietrzu na stanowiskach pracy z zastosowaniem chromatografii gazowej z detekcją mas. Metoda polega na adsorpcji bromoetanu w rurce, wypełnionej...
Ethyl carbamate. Documentation of proposed values of occupational exposure limits (OELs)
Ethyl carbamate (urethane, CAS 51-79-6) is a solid, odorless and soluble in water and organic solvents. In an environment it occurs as a natural product produced during alcoholic fermentationof foods and beverages contai...