A A VALIDATED REVERSE-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY-CHARGED AEROSOL DETECTOR TECHNIQUE FOR THE SIMULTANEOUS ESTIMATION OF SITAGLIPTIN AND ERTUGLIFLOZIN IN PURE AND PHARMACEUTICAL DOSAGE FORMS
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2019, Vol 12, Issue 5
Abstract
Objective: The main objective of the present work is to develop and validate a selective reverse-phase (RP) high-performance liquid (HPLC)-charged aerosol detection technique for the quantitation of the sitagliptin and ertugliflozin in dosage form to attain high degree of sensitivity. Materials and Methods: In present HPLC technique, separation of drugs was achieved on Phenomenex C18 column (250×4.6 mm, 5 μ) with a mobile phase composition of phosphate buffer (pH – 5.8), acetonitrile, and methanol in the proportion of 40:40:20%V/V. 1 ml/min flow rate and 256 nm wavelength detection were maintained for the elution of drugs in the chromatographic system. The retaining time of sitagliptin and ertugliflozin in column was found to be 4.2 and 2.4 min, respectively. Results: The projected technique was successfully applied for the quantitation of sitagliptin and ertugliflozin as a single combined mixture. The linearity statistics for calibration curves shown a good linearity in the concentration range of 0.3125–10 μg/ml for sitagliptin and 0.0625–2.5 μg/ml for ertugliflozin. The average values of regression coefficient, slope, and intercept were 0.9998, 8688.2, and 1977.6 for sitagliptin and 0.9996, 33602, and 1852.6 for ertugliflozin. The technique was validated as per the International Council for Harmonization guidelines. The limit of detection and limit of quantification findings were 0.082 and 0.247 μg/ml for sitagliptin and 0.04 and 0.12 μg/ml for ertugliflozin. Conclusion: The developed and validated RP-HPLC-charged aerosol detector technique of sitagliptin and ertugliflozin in dosage form showed that the method was accurate and selective with high degree of sensitivity.
Authors and Affiliations
ANJANEYULU REDDY B , RADHAKRISHNANAND P , IRSHAD ALAM MD , RAVI KIRAN P
Keywords
Related Articles
- EP ID EP604748
- DOI -
- Views 107
- Downloads 0
ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF TRANS-CINNAMALDEHYDE NANOEMULSION AGAINST ESCHERICHIA COLI
Objective: Trans-cinnamaldehyde (TC) has shown antimicrobial activity against various microorganisms, but its direct use has some disadvantages such as skin irritation, low bioavailability, and low solubility. The object...
NANOSTRUCTURED LIPID CARRIERS: THE FRONTIERS IN DRUG DELIVERY
By the modification of solid-lipid nanoparticles (SLNs), the nano-structured lipid carriers (NLCs) were obtained by the modification of nanostructured that hold the qualities of the SLN, increases the stability of the dr...
THERAPEUTIC VALUE OF ARCTIUM LAPPA LINN. – A REVIEW
Arctium lappa (AL) (Burdock) has been used for centuries to treat a variety of ailments. Extracts of burdock root (BR) are found in a variety of herbal preparations, as well as homeopathic remedies. Arctium has been used...
A STUDY OF URINE FROM HIV-POSITIVE AND HIV-NEGATIVE INDIVIDUALS AT ANDHRA PRADESH
Objective: The aim of this study is to investigate the variations, if any, in urinary bacterial pathogens in HIV-positive and HIV-negative individuals.Methods: Urine sample, macroscopic and microscopic examination for id...
EFFECT OF MANGOSTEEN EXTRACT ON NEUROINFLAMMATION IN RAT MODEL OF ACUTE TRAUMATIC BRAIN INJURY
Objective: Traumatic brain injury (TBI) is one of the major health problems regarding morbidity and mortality, especially in productive ages. Following primary injury, there is a secondary insult, resulting in oxidative...